摘要
ANKTM(Ankyrin repeats transmembrane)蛋白属锚蛋白超家族,包含ANK基序和跨膜结构域,在非生物胁迫、生物胁迫、诱发衰老中起重要作用。为了解大豆ANKTM(Ankyrin repeats transmembrane)家族成员及其胁迫响应规律,利用生物信息学和q-PCR方法研究大豆ANKTM家族成员。结果表明,从大豆中鉴定出62个ANKTM家族基因,分为5个亚家族。该家族编码蛋白含346~1 039个氨基酸,分子质量为37.88~114.83 ku,等电点(pI)为4.46~9.61。分析蛋白结构域发现,GmANKTM家族含ANK基序和跨膜结构域,58个基因含PGG结构域,此外还含ACBP、SBP、DHHC、G-PCR等其他结构域。除ANK基序、跨膜结构域和PGG结构域外其他结构域主要集中在第5亚家族。其余亚家族结构域分布位置和数量相近。启动子区域顺式元件分析发现,GmANKTM家族基因含光响应元件、激素响应相关元件和逆境响应相关元件。组织分析发现GmANKTM家族第5亚家族组织表达量高于其他亚家族。利用大豆转录组数据库分析发现,GmANKTM家族基因受盐胁迫和干旱胁迫强烈诱导。
ANKTM (Ankyrin repeats transmembrane) protein belongs to the ankyrin superfamily and contains ANK motif and transmembrane domain, which plays an important role in abiotic stress, biotic stress and induced senescence. In order to understand the members of soybean ANKTM (Ankyrin repeats transmembrane) family and their functional roles in stress response rules, the bioinformatics and qPCR methods were used to study the soybean ANKTM family members.The results showed that 62 ANKTM family genes were identified from soybeans and divided into five subfamilies. The family protein contained amino acids which were form 346 to 1 039. The molecular weight was from 37.88 to 114.83 ku. An isoelectric point (pI) was ranging from 4.46 to 9.61. Analysis of the protein domain revealed that the GmANKTM family contained an ANK motif and a transmembrane domain, and 58 genes contained a PGG domain, in addition to other domains, such as ACBP, SBP, DHHC, and GPCR. Other domains except the ANK motif, the transmembrane domain, and the PGG domain were mainly concentrated in the fifth subfamily. The remaining subfamily domains were located in similar locations and numbers. The cis- element analysis of the promoter region revealed that the GmANKTM family of genes contained photoresponsive elements, hormone response-related elements, and stressresponsive elements. Tissue analysis revealed that the fifth subfamily of the GmANKTM family expressed higher in tissues than the other subfamilies. Using the soybean transcriptome database analysis, it could be found that the GmANKTM family genes were strongly induced by salt stress and drought stress.
作者
柏锡
王昕奕
陈云
杨亚男
黄荣梅
郑佳佳
王佳
BAI Xi;WANG Xinyi;CHEN Yun;YANG Yanan;HUANG Rongmei;ZHENG Jiajia;WANG Jia(School of Life Sciences, Northeast Agricultural University, Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2019年第7期18-27,39,共11页
Journal of Northeast Agricultural University
基金
转基因生物新品种培育重大专项(2016ZX08004002)
