摘要
目的:研究黄芩苷对支气管哮喘防治的作用及其可能的作用机制。方法:将54只SD大鼠按体质量随机分为正常组、哮喘模型组、地塞米松组及黄芩苷高、低剂量组。用卵清蛋白(OVA)诱发致敏大鼠建立支气管哮喘的动物模型,给予相应药物灌胃给药4周,1次/d,其中正常组及哮喘模型组药物以等量生理盐水代替。用药物干预性治疗后,观察各组药物对大鼠的哮喘程度及哮喘潜伏期的影响。实验结束后,24h内处死大鼠,取材,测定并比较支气管壁厚度、气道上皮下胶原沉积程度;测定血中HMGB1浓度;免疫组织化学测定支气管平滑肌中α-SMA和气道组织TLR4的蛋白表达;RT-PCR法测定肺组织HMGB1mRNA表达变化。结果:黄芩苷可明显改善病鼠的哮喘程度,延长哮喘潜伏期(P<0.05~0.01);可有效减轻病鼠支气管管壁增厚及气道上皮下胶原沉积程度(P<0.05);明显降低血浆中HMGB1的含量,肺组织中的HMGB1mRNA表达呈下降趋势,但差异无统计学意义(P>0.05);可明显降低气道组织中的TLR4,气管平滑肌中α-SMA的蛋白表达及TLR4mRNA的表达水平(P>0.05)。结论:黄芩苷具有抗哮喘作用,其作用可能与其影响HMGB1/TLR4通路的信号传导相关。
Objective: To study the effects of Baicalin on prevention and treatment of bronchial asthma and its possible mechanism. Methods: A total of 54 SD rats were randomly divided into a normall group,an asthma model group,a dexamethasone group,baicalin high and low dose groups by weight.Animal model of bronchial asthma was induced by ovalbumin (OVA).The corresponding drugs were given by orally intragastric administration once a day for 4 weeks.The normal group and the asthma model group were replaced by same volume of normal saline (NS).The effects on asthmatic degree and the latent period of asthma of each rats group were observed after treatment.After the experiment,the rats were killed within 24 h for collection of materials.The thickness of bronchial wall,the degree of collagen deposition in airway,the concentration of HMGB1 in blood,the expression of α-SMA in bronchial smooth muscle and TLR4 in airway were measured by immunohistochemistry.The expression of HMGB1 mRNA in lung tissue was determined by RT-PCR method. Results: Baicalin high and low dose groups could obviously improve the asthmatic degree and prolong the latent period of asthma ( P <0.05-0.01),and could effectively reduce the thickness of bronchial wall and the degree of subcutaneous collagen deposition in airway ( P <0.05).The content of HMGB1 in plasma was significantly decreased,and the expression of HMGB1 mRNA in lung tissue was decreased,but there was no significant difference ( P >0.05).The protein expression of α-SMA and TLR4,the expression of TLR4 mRNA in tracheal smooth muscle were significantly decreased ( P >0.05). Conclusion: Baicalin has an effect of anti-asthmatic,which may be related to its influence on signal transduction of the HMGB1/TLR4 pathway.
作者
韩超
杨柳
张秋玲
梁慧玲
徐俊
Han Chao;Yang Liu;Zhang Qiuling;Liang Huiling;Xu Jun(Guangzhou Hospital of Chinese Medicine,Guangzhou 510130,China;College of Pharmacy,Jinan University,Guangzhou 510632,China)
出处
《世界中医药》
CAS
2019年第6期1402-1407,共6页
World Chinese Medicine
基金
广东省中医药局基金项目(20141226,20172A010009)
广州市中医药、中西医结合项目(20142A011001)
