摘要
为了明确抗根肿病大白菜材料G6所含抗病基因,开发连锁标记,以高感根肿病的大白菜高代自交系G4、高抗根肿病的大白菜高代自交系G6、G4和G6杂交得到的F1及F1自交构建的F2分离群体为材料,通过人工接种、表型鉴定和遗传学分析,发现该抗病材料中的根肿病抗性由显性单基因控制。进一步扩大F2群体数量对根肿病抗性基因的初定位,筛选与其连锁的分子标记,利用JoinMap4.0软件对多态性标记进行连锁分析,获得了5个与大白菜抗根肿病基因连锁的InDel标记,其中最近的两侧标记为BrID10727和BrID10867,与抗病基因的遗传距离分别为4.6,2.5cM,抗病基因定位在大白菜染色体A08上。此外,经验证发现基于Crr1基因序列新开发的多态性标记BrID10381不在新定位抗根肿基因初定位区间内,因此,可以推断新定位抗根肿基因位点与Crr1并非同一位点,且标记BrID10381可以用于Crr1基因的分子标记辅助选择。
In order to identify the clubroot resistance gene of Chinese cabbage in G6 and develop linkage markers,high-generation inbred line G4 of Chinese cabbage with high-susceptible to clubroot disease,high-generation inbred line G6 of Chinese cabbage with high-resistant to clubroot disease,F1 obtained by crossing G4 and G6,and the F 2 generation separation populations constructed by F1 selfing were used as materials. By artificial inoculation,phenotypic identification and genetic analysis,it was found that clubroot disease resistance in the disease resistance materials were controlled by a dominant single gene. Further expanding the number of F 2 generation populations to map the genes of clubroot disease and screening molecular markers linked to disease resistance genes,the linkage analysis of polymorphic markers was performed using JoinMap 4.0 software,and five InDel markers linked to the resistance gene of Chinese cabbage were obtained. The most recent markers on both sides were BrID10727 and BrID10867,the genetic distance from the disease resistance genes were 4.6,2.5 cM,respectively,and the disease resistance gene was located on the chromosome A08 of Chinese cabbage. In addition,it was found that the newly developed polymorphic marker BrID10381 based on the Crr1 gene sequence was located outside the initial localization range of the newly located clubroot resistance gene,so it could be inferred that the newly located clubroot resistance gene locus was not the same locus as Crr1,and the marker BrID10381 could be used for molecular marker assisted selection of Crr1 gene.
作者
王超楠
张斌
张红
温娟娟
王涛
WANG Chaonan;ZHANG Bin;ZHANG Hong;WEN Juanjuan;WANG Tao(Tianjin Kernel Vegetable Research Institute,Key Laboratory of Tianjin Vegetable Geneticand Breeding Enterprises,State Key Laboratory of Vegetable Germplasm Innovation,Tianjin 300384,China;College of Life Sciences,Tianjin Normal University,Tianjin 300387,China)
出处
《华北农学报》
CSCD
北大核心
2019年第3期203-208,共6页
Acta Agriculturae Boreali-Sinica
基金
国家重点研发计划(2017YFD0101801)
天津市农业科学院青年科研人员创新研究与实验项目(2018003
2018013)
天津市现代农业产业技术体系创新团队建设专项计划(ITTVRS2017003)
关键词
大白菜
根肿病
基因定位
连锁标记
Chinese cabbage
Clubroot disease
Gene mapping
Linkagemarker
