摘要
本文用腺嘌呤建立肾脏和睾丸损伤的小鼠模型,探索蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成的肾脏和睾丸损伤的恢复作用。将小鼠随机分为空白组、模型组和蝙蝠蛾拟青霉菌Cs-4菌粉的5个剂量组(350mg/kg、550mg/kg、750mg/kg、1 500mg/kg、3 000mg/kg),连续给予相应药物21d,对各组小鼠的肾脏、睾丸通过HE染色、Masson染色,进行组织病理学检查,测定各组小鼠血清中尿素氮(BUN)、肌酐(Cr)、促卵泡刺激素(FSH)、黄体生成素(LH)含量,测定睾丸组织匀浆中的酸性磷酸酶(ACP)、琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)水平。结果表明,蝙蝠蛾拟青霉菌Cs-4菌株具有使小鼠体重逐渐恢复上升趋势、使受损的肾脏和睾丸组织结构恢复的作用,能使BUN、Cr、FSH、LH、ACP、SDH和LDH水平趋于正常化。
A mouse model of kidney and testis injury was established using adenine to explore the recovery effect of fermented powder products of Paecilomyces hepiali Cs-4 on adenine-induced kidney and testis damage. The mice were randomly divided into control group, model group, five treatment groups orally treated with different doses of fermented powder products of Paecilomyces hepiali Cs-4(350 mg/kg, 550 mg/kg,750 mg/kg, 1 500 mg/kg, 3 000 mg/kg). The mice were treated daily for consecutive 21 days. The histopathological observation of the kidney and testis of each group of mice was carried out by using HE and Masson staining.The content of BUN, Cr, FSH and LH in blood serum were detected. In addition, the content of lactate dehydrogenase(LDH), acid phosphatase(ACP) and succinate dehydrogenase(SDH) in testis homogenate were detected. The results showed that the body weight of mice gradually recovered and increased in the experimental group treated with fermented powder products of Paecilomyces hepiali Cs-4, manifesting a recovery effect on damaged kidney and testis structure, and normalization of BUN, Cr, FSH, LH, ACP, SDH and LDH levels.
作者
闫俊
包海鹰
闫芳
杨明
图力古尔
李玉
YAN Jun;BAO Hai-Ying;YAN Fang;YANG Ming;BAU Tolgor;LI Yu(Key Laboratory of Medicinal Fungal Resources and Development and Utilization,Jilin Agricultural University,Changchun, Jilin 130118,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118,China;Jiangxi Guoyao Co., Ltd, Nanchang, Jiangxi 330052,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第6期926-937,共12页
Mycosystema
基金
国家中药标准化项目—金水宝胶囊标准化建设(ZYBZH-C-JX-39)~~
关键词
蝙蝠蛾拟青霉菌Cs-4菌株
腺嘌呤
肾脏
睾丸
恢复作用
fermented powder products of Paecilomyces hepiali Cs-4
adenine
kidney
testis
reproductive function
