摘要
通过研究小分子前体物对巴弗洛霉素A1 生物合成的影响,寻找提高巴弗洛霉素A1 发酵产量的有效方法。主要考察了不同前体物对巴弗洛霉素A1 生物合成的影响,以及有效前体物的最佳添加浓度和添加时间点。结果显示,缬氨酸是巴弗洛霉素A1 合成的最佳前体,36 h 时添加0.2%缬氨酸,巴弗洛霉素A1 产量高达285.5 mg/L,比对照组提高了78.4%。前体物的供给能显著影响巴弗洛霉素A1 的生物合成,补加缬氨酸能有效提高巴弗洛霉素A1 的发酵产量。
The objective of this work is to explore an effective method of increasing bafilomycin A1 productivity via understanding the effects of small molecule precursors on bafilomycin A1 biosynthesis. The effects of different precursors on the biosynthesis of bafilomycin A1,the optimal concentration and feeding time of the effective precursor were mainly studied. As result,valine was the optimal precursor for bafilomycin A1 biosynthesis. By feeding of 0.2% valine into the fermentation broth at the 36th hour of cultivation,the bafilomycin A1 production reached 285.5 mg/L,which was 78.4% higher than that in the condition without any addition of precursor. In conclusion,feeding precursors significantly influences bafilomycin A1 biosynthesis,and the addition of valine is effective in increasing bafilomycin A1 production.
作者
周剑
孙菲
方志锴
江红
ZHOU Jian;JIANG Hong;SUN Fei;FANG Zhi-kai(Fujian Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology)
出处
《生物技术通报》
CAS
CSCD
北大核心
2019年第6期125-130,共6页
Biotechnology Bulletin
基金
国家重点研发计划(2018YFC0311004)
国家“十三五”重大新药创制项目(2018ZX09101003-007)
福建省公益类科研院所省重点项目(2019R11020006-6)
