摘要
目的:探讨芍药汤对湿热内蕴型溃疡性结肠炎(UC)大鼠疾病活动指数及血清中白介素8(IL-8)的影响。方法:将60只健康Wistar大鼠按照随机数字表法分为正常对照组、模型对照组、芍药汤高、中、低剂量组、柳氮磺吡啶(SASP)组。湿热内蕴型溃疡性结肠炎模型大鼠应用高脂高糖辛辣饮食加免疫复合法+TNBS(2,4,6-三硝基苯磺酸)结合乙醇方法复制。按照IL-8ELISA试剂盒操作说明测定血清中的IL-8水平。在实验开始后24小时、造模成功后24小时、给药治疗后20天对各组大鼠进行疾病活动指数(DAI)评价。结果:DAI:在造模成功后24小时,空白对照组与其余各组相比较疾病活动指数差异有统计学意义(P<0.05)。在治疗后第20天时,空白对照组与各组相比差异有统计学意义(P<0.05),模型对照组与不同剂量的芍药汤组和SASP组对比具差异有统计学意义(P<0.05)。各组大鼠血清中IL-8含量:与正常对照组相比,模型对照组大鼠血清中IL-8含量明显升高,具有统计学意义(P<0.05)。与模型对照组相比较,各剂量芍药汤组、SASP组大鼠血清IL-8含量明显降低,组间比较差异有统计学意义(P<0.05)。结论:芍药汤可降低IL-8的含量,降低组织中炎性细胞含量,并加快对肠黏膜修复的作用。
Objective:To explore the effects of ShaoYao decoction on disease activity indexes and the levels of IL-8 of the rats with ulcerative colitis(UA)of damp-heat internal accumulation pattern.Methods:Sixty rats were divided into normal control group,model control group,high,moderate and low dose groups of ShaoYao decoction,SASP group.rat models suffering UC of damp-heat internal accumulation pattern were replicated by applying high-fat high-sugar acrid diet and immune complex method+TNBS combined with alcohol method.The levels of IL-8 were detected on the basis of the operation instruction of IL-8 ELISA kit.DAI of the rats in different groups were assessed over 24 hours after the experiment began,24 hours after successfully establishing the models and 20 days after treating.Results:DAI:Over 24 hours after successfully establishing the models,statistical difference was found in DAI among blank control group and others groups(P<0.05).Over 20 days after treating,the differences were statistically significant when blank control group was compared with the others(P<0.05).Statistical differences were found when model control group was compared with different dose groups of ShaoYao decoction and SASP group(P<0.05).The contents of IL-8 in the rats of different groups:Compared with normal control group,the contents of IL-8 raised notably in the rats of model control group,and the difference had statistical meaning(P<0.05).Compared with model control group,the contents of IL-8 lowered obviously in different dose groups of ShaoYao decoction and SASP group,the differences had statistical meaning(P<0.05).Conclusion:ShaoYao decoction could decrease the contents of IL-8,reduce the contents of inflammatory cells in the tissue and accelerate the recovery of intestinal mucosa.
作者
祖健
赵党生
王凤仪
张小元
徐兰萍
卢晓婷
何小磊
王移飞
蒲晓薇
ZU Jian;ZHAO Dangsheng;WANG Fengyi;ZHANG Xiaoyuan;XU Lanping;LU Xiaoting;HE Xiaolei;WANG Yifei;PU Xiaowei(Gansu University of Chinese Medicine,Lanzhou 730000,China;Affiliated Hospital of Gansu University of Chinese Medicine)
出处
《西部中医药》
2019年第4期29-32,共4页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金(编号81560746)
