摘要
目的:研究两株乳酸菌对糖尿病小鼠血糖、血脂、血清炎症因子、肝脏抗氧化水平、胰腺病理变化和附睾脂肪组织白细胞介素-10(interleukin-10,IL-10)及脂联素(adiponectin,Adipoq)基因表达的影响。方法:通过降胆固醇、耐酸及耐胆盐等体外实验从6株乳酸菌中筛选降脂较好的2株乳酸菌。利用链脲佐菌素(streptozotocin,STZ)结合高糖高脂饲料的方法建立小鼠糖尿病模型。将100只雄性BALB/c小鼠随机分为4组,每组25只,对照组(N)(正常饮食)、模型组(M)(高糖高脂饮食)、SY13菌组(SY13)(高糖高脂饮食外加SY13菌液灌胃)、36号菌组(D36)(高糖高脂饮食外加36号菌液灌胃),分别在灌胃6、10周和14周时处死小鼠,动态检测小鼠血糖、血脂等相关生化指标水平,利用苏木精-伊红染色观察胰腺组织病理变化,通过逆转录实时荧光定量聚合酶链式反应方法检测附睾脂肪组织中IL-10和Adipoq基因的m RNA表达情况。结果:筛选出干酪乳酸菌SY13和D36有较强的降胆固醇、耐酸和耐胆盐能力。与M组相比,通过一段时间菌液的灌胃,显著抑制了小鼠血糖水平和血清中总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平的上升(P<0.05),提高了高密度脂蛋白胆固醇水平和抗炎因子IL-10水平(P<0.05),不同程度提高了肝脏谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶活力,显著降低了丙二醛含量(P<0.05),改善了小鼠糖代谢紊乱状况,明显减轻了胰岛和腺泡细胞病变情况,脂肪组织中IL-10和Adipoq基因表达水平显著提高。结论:乳酸菌SY13和D36能够显著改善糖高脂饮食联合STZ诱导的小鼠糖脂代谢。
Objective: To study the effects of two Lactobacillus strains on blood glucose, blood lipids, serum inflammatory cytokines, liver antioxidant level, pancreatic pathology, and the expression of the genes encodig interleukin-10 (IL-10) and adiponectin (adipoq) in epididymal adipose tissue in diabetic mice. Methods: Out of six Lactobacillus strains, two Lactobacillu casei strains (SY13 and D36) were selected for their potent lipid-lowering effect in terms of cholesterollowering ability, and acid and bile salt tolerance in vitro. The diabetic mouse model was established by feeding mice a highglucose and high-fat diet and injecting them with streptozotocin (STZ). Totally 100 male Balb/c mice were randomly divided into four groups of 25 mice per group: control, model, SY13 and D36 groups. The control group was fed a normal diet, while all the other groups were given a high-glucose and high-fat diet. The SY13 and D36 groups were additionally administered by gavage with Lactobacillu casei SY13 and D36, respectively. Some of the mice were scarified at the end of the sixth, tenth and fourth week of administration for the detection of blood glucose, blood lipids and other related biochemical indexes. HE staining was used to observe the pathological change of pancreatic tissues, and the mRNA expression of IL-10 and Adipoq genes in epididymal adipose tissues were detected by reverse transcription quantative real-time polymerase chain reaction. Results: L. casei SY13 and D36 had high cholesterol-lowering capacity, and strong acid and bile salt tolerance. Compared with the model group, both strains significantly repressed the increase in blood sugar and serum total cholesterol, triglyceride and low-density lipoprotein cholesterol levels, and increased the levels of serum high-density lipoprotein chrolestserol and anti-inflammatory factor interleukin-10 (IL-10)(P < 0.05). In addition, they significantly increased the activity of glutatione peroxidase, superoxide dismutase and catalase in liver and decreased malondialdehyde (P < 0.05). The strains also improved glucose metabolism disorders, obviously alleviated the pathological changes of islet and adenocytic cells, and promoted the mRNA expression of IL-10 and Adipoq genes in adipose tissues. Conclusion: Lactobacillus casei SY13 and D36 can significantly improve glucose and lipid metabolism in mice with diabetes induced by a high-glucose and high-fat diet combined with STZ injection.
作者
高伟华
刘鹭
王芬
逄晓阳
张书文
芦晶
段江燕
吕加平
GAO Weihua;LIU Lu;WANG Fen;PANG Xiaoyang;ZHANG Shuwen;LU Jing;DUAN Jiangyan;Lü Jiaping(College of Life Science, Shanxi Normal University, Linfen 041000,China;Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193,China;Engineering Research Centre of System-Nutrition, Beijing Institute of Nutrition Resources, Beijing 100069,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第9期179-187,共9页
Food Science
基金
农业部中央级公益性科研院所基本科研业务费项目(S2016JC01)
关键词
乳酸菌
高糖高脂饮食
血脂
抗炎因子
抗氧化活性
Lactobacillus
high-glucose and high-fat diet
serum lipids
anti-inflammatory factor
antioxidant activity
