摘要
目的观察加味玄麦甘桔浸膏的抗炎、祛痰、抑菌作用。方法采用孙氏改良寇氏法测定半数致死量(LD50);二甲苯致小鼠耳肿胀和棉球致肉芽肿模型研究抗炎作用;小鼠酚红排泌实验观察祛痰活性;体外抑菌实验评价对金黄色葡萄球菌、铜绿假单胞菌、肺炎链球菌等常见上呼吸道致病菌的抑菌作用;测定急性咽炎模型中大鼠血常规指标的变化和血清中肿瘤坏死因子(TNF)和白细胞介素-1 (IL-1)的水平。结果加味玄麦甘桔浸膏LD50值为30.71 g·kg^(-1);不同浓度加味玄麦甘桔浸膏(0.77,1.54,3.07 g·kg^(-1))对急慢性炎症模型均具有明显的抗炎作用,并呈剂量依赖关系;酚酞祛痰实验显示,不同剂量加味玄麦甘桔浸膏均可增强气管酚红的排出;急性咽炎模型大鼠血浆中白细胞计数、中性粒细胞和淋巴细胞百分比含量和血清中TNF、IL-1水平均降低。结论加味玄麦甘桔浸膏具有显著的抗炎、祛痰、抑菌作用,其作用机制可能是抑制TNF及IL-1通路。
Objective To observe the effect of the extract of Jiawei Xuanmai Ganjie on pharyngitis of anti-inflammatory, expectorant and antibacterial. Methods The LD 50 was determined by Sun's modified Karber's method. To study the anti-inflammatory effects of the extract, dimethylbenzene on ear swelling and cotton granulomatous model in mice was established. The phlegm removing activity in mice was observed with phenolic red excretion. The antibacterial effect on Staphylococcus aureus , Pseudomonas aeruginosa and Streptococcus pneumoniae , the common pathogenic bacteria in throat and upper respiratory tract, was evaluated by in vitro antimicrobial test. At the same time, the routine blood test and the levels of tumor necrosis factor (TNF) and interleukin-1 (IL-1) in serum were measured in rat acute pharyngitis model. Results The LD 50 value of extract was 30.71 g·kg ^-1 . The extract had obvious anti-inflammatory effects on acute and chronic inflammation models in a dose-dependent manner (0.77, 1.54 and 3.07 g·kg^-1 ). In the phenolphthalein expectorant test, the extract of different doses could enhance the excretion of phenol red in trachea. In rat acute pharyngitis model, the percentage of white blood cells, neutrophils and lymphocytes in plasma and the levels of TNF and IL-1 in serum significantly decreased in the extract-treated group. Conclusion The extract of Jiawei Xuanmai Ganjie has significant anti-inflammatory, expectorant and bacteriostasis effects on pharyngitis, which may exert its effect by inhibiting TNF and IL-1 pathways.
作者
申玲玲
杜光
SHEN Lingling;DU Guang(Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China)
出处
《医药导报》
CAS
北大核心
2019年第5期563-567,共5页
Herald of Medicine
关键词
加味玄麦甘桔浸膏
抗炎作用
祛痰作用
抑菌作用
Extract of Jiawei Xuanmai Ganjie
Anti-inflammatory
Expelling phlegm
Anti-bacterial effect
