摘要
Angelicae Pubescentis Radix (APR, Duhuo) is a commonly used traditional Chinese medicine and usually used for the treatments of rheumatic diseases. To assess the chemical composition of APR extract, a sensitive and reliable UPLC-Q?TOF-MS method was used for qualitative analysis. The separation was achieved on an Agilent SB-Ci8 column (1.8 pm, 2.1 mm><50 mm) with a gradient elution system consisting of acetonitrile and water containing 0.1% formic acid. An electrospray ionization (ESI) was used for mass spectrometer, and the data were collected in the positive ion mode, which was operated in a full-scan mode at m/z 100-800. A total of 49 compounds including 46 coumarins were identified according to the MS and MS/MS data. Among them, two were new compounds, and isoangenomalin, scoparone, 4-methyl-umbelliferyl acetate, suberenol,/raw5-dehydroosthol, and oroselone were first reported in APR.
独活(Angelicae Pubescentis Radix)作为一种被广泛应用的中药,常用于治疗风湿性疾病。本研究通过运用UPLC-Q-TOF-MS液质联用技术定性分析独活提取物中的化学成分。独活提取物的分离通过Agilent SB-C_(18)色谱柱(1.8μm,2.1 mm×50 mm)经梯度洗脱完成,流动相由0.1%甲酸–水和乙腈组成。通过配有ESI电离源的质谱仪在m/z 100–800全扫描正离子模式下进行数据采集。通过比较一级和二级质谱数据,共鉴定出49个化合物,包括46个香豆素类和3个非香豆素类化合物。其中2个化合物被鉴定为新化合物,isoangenomalin,scoparone,4-methyl-umbelliferyl acetate,suberenol,trans-dehydroosthol和oroselone为首次在独活中发现的化合物。
基金
National Nature Science Foundation of China(Grant Nos.81503208,81403068,30672609)
Beijing Municipal Natural Science Foundation(Grant No.7144219)
National Key Technology R&D Program of China(Grant No.2011BAI07B08)
