摘要
目的建立GC法同时测定小儿豉翘清热颗粒(淡豆豉、连翘、黄芩等)中α-蒎烯、β-蒎烯、月桂烯、柠檬烯、芳樟醇、薄荷酮、薄荷脑、胡薄荷酮、柠檬醛的含有量。方法该药物乙酸乙酯提取液的分析采用DB-1色谱柱(30 m×0.25 mm,0.25μm),程序升温;体积流量0.5 mL/min;进样口温度230℃;检测器温度280℃;分流比30∶1。结果 9种挥发性成分在各自范围内线性关系良好(r≥0.998 7),平均加样回收率96.39%～102.2%,RSD 1.3%～2.8%。结论该方法简便、准确、可靠,可用于小儿豉翘清热颗粒的质量控制。
AIM To establish a GC method for the simultaneous content determination of α-pinene,β-pinene,myrcene,limonene,linalool,menthone,menthol,pulegone and citral in Xiao’er Chiqiao Qingre Granules(Sojae Semen Praeparatum,Forsythiae Fructus,Scutellariae Radix,etc.).METHODS The analysis of ethyl acetate extract of this drug was performed on a temperature-programmed DB-1 column(30 m×0.25 mm,0.25 μm)flowing at 0.5 mL/min with the split ratio of 30:1,and the inlet temperature and detector temperature were set at 230 ℃ and 280 ℃,respectively.RESULTS Nine volatile constituents showed good linear relationships within their own ranges(r≥0.998 7),whose average recoveries were 96.39%-102.2% with the RSDs of 1.3%-2.8%.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Xiao’er Chiqiao Qingre Granules.
作者
李超
田刚
吴菲
朱法根
刘雪
石心红
封亮
贾晓斌
LI Chao;TIAN Gang;WU Fei;ZHU Fa-gen;LIU Xue;SHI Xin-hong;FENG Liang;JIA Xiao-bin(Jumpcan Pharmaceutical Group Co.,Ltd.,Taixing 225400,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第4期739-742,共4页
Chinese Traditional Patent Medicine
基金
国家中医药管理局标准化建设项目(ZYBZH-C-JS-33)
江苏省博士后科研资助计划项目(1601023C)
中国博士后科研资助计划项目(2016M601864)
中医药现代化研究重点专项(2018YFC1706900)
中国药科大学双一流创新团队(CPU2018GF07
CPU2018GY11)
关键词
小儿豉翘清热颗粒
挥发性成分
GC
Xiao'er Chiqiao Qingre Granules
volatile constituents
GC
