摘要
目的评价趋化因子CXC配体16(CXCL16)在肾缺血再灌注损伤小鼠肾纤维化中的作用。方法健康雄性C57BL/6小鼠24只,8~10周龄,体重20~30g。采用随机数字表法分为4组(n=6):假手术组(S组)、假手术+抗CXCL16抗体组(S+A组)、缺血再灌注组(I/R组)和缺血再灌注+抗CXCL16抗体组(I/R+A组)。采用阻断左侧肾动脉30min后再灌注,第5天时切除右侧肾脏的方法制备肾缺血再灌注损伤模型。I/R+A组和I/R组分别于再灌注后72h腹腔注射抗CXCL16抗体5mg/kg和等容量生理盐水,每周2次,连续2周;S+A组和S组实施假手术,分别于相应时点腹腔注射等量抗CXCL16抗5mg/kg体和等容量生理盐水。再灌注14d时采集眼眶静脉血样,检测血清BUN和Cr浓度;然后处死小鼠,取左侧肾组织,采用天狼星红染色法测定肾纤维化面积,采用Western blot法检测α-平滑肌肌动蛋白(α-SMA)、纤连蛋白(FN)和Ⅰ型胶原蛋白(Col-Ⅰ)的表达。结果与S组比较,I/R组血清BUN和Cr浓度升高,肾纤维化面积增加,肾组织α-SMA、FN和Col-Ⅰ表达上调(P<0.05);与I/R组比较,I/R+A组血清BUN和Cr浓度降低,肾纤维化面积降低,肾组织α-SMA、FN和Col-Ⅰ表达下调(P<0.05)。结论CXCL16参与了肾缺血再灌注损伤小鼠肾纤维化的过程。
Objective To evaluate the role of chemokine CXC-ligand 16(CXCL16)in renal fibrosis following renal ischemia-reperfusion(I/R)injury in mice.Methods Twenty-four healthy male C57BL/6 mice,aged 8-10 weeks,weighing 20-30 g,were divided into 4 groups(n=6 each)using a random number table method:sham operation group(group S),sham operation plus anti-CXCL16 antibody group(group S+A),group I/R,and I/R plus anti-CXCL16 antibody group(group I/R+A).Renal ischemia was induced by occlusion of the left renal artery for 30 min followed by 72-h reperfusion,and the right kidney was removed on 5th day in anesthetized mice.CXCL16 antibody 5 mg/kg and the equal volume of normal saline were intraperitoneally injected at 72 h after reperfusion twice a week for 2 consecutive weeks in I/R+A and I/R groups,respectively.Sham operation was performed,and the equal volume of CXCL16 antibody 5 mg/kg and normal saline were intraperitoneally injected at 72 h after reperfusion twice a week for 2 consecutive weeks in S+A and S groups,respectively.Orbital venous blood samples were collected at day 14 after reperfusion for determination of serum blood urea nitrogen(BUN)and creatinine(Cr)concentrations.Animals were then sacrificed and the left kidney was removed for measurement of the renal fibrosis size(using Sirius red staining),expression of α-smooth muscle actin(α-SMA),fibronectin(FN)and type Ⅰ collagen(Col-Ⅰ)in renal tissues by Western blot.Results Compared with group S,the serum BUN and Cr concentrations and renal fibrosis size were significantly increased,and the expression of α-SMA,FN and Col-Ⅰ was up-regulated in group I/R(P<0.05).Compared with group I/R,the serum BUN and Cr concentrations and renal fibrosis size were significantly decreased,and the expression of α-SMA,FN and Col-Ⅰ was down-regulated in group I/R+A(P<0.05).Conclusion CXCL16 is involved in the process of renal fibrosis following renal I/R injury in mice.
作者
徐枫
尹美艳
张涛
梁桦
廖美娟
贺俭
Xu Feng;Yin Meiyan;Zhang Tao;Liang Hua;Liao Meijuan;He Jian(Department of Anesthesiology,Affiliated Foshan Hospital,Sun Yat-Sen University,Foshan 528000,China;Department of Otolaryngology,Affiliated Foshan Hospital,Southern Medical University,Foshan 528000,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第11期1376-1379,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(81871539)
广东省自然科学基金(2018A030313613)
佛山市卫生和计生局医学科研课题(20170021)。
