摘要
目的:探讨地塞米松对急性胰腺炎(acute pancreatitis,AP)小鼠胰腺组织中Notch1,Hes1及凋亡因子Bax、Bcl-2表达的影响。方法:利用蛙皮素建立小鼠急性胰腺炎动物模型,实验分组为生理盐水组(对照组),模型组(AP组),地塞米松(dexamethasone,DEX)组,利用ELISA法检测各组动物血清中TNF-α, IL-6的含量;利用实时荧光定量PCR(RT-qPCR)检测各组动物胰腺组织中Notch1,Hes1,Bax及Bcl-2 mRNA的表达情况;利用Western blot检测各组胰腺组织中Notch1,Hes1,Bax及Bcl-2蛋白的表达情况。结果:ELISA检测结果显示,与模型组相比较,DEX组动物血清中TNF-α与IL-6的含量显著降低;RT-qPCR与Western blot结果显示,与模型组相比较,DEX组胰腺组织中Notch1,Hes1,Bcl-2 mRNA的表达与蛋白的表达均显著降低,而Bax mRNA的表达与蛋白的表达显著升高。结论:DEX可能通过抑制Notch信号的激活,抑制Notch1与Hes1的表达,进一步抑制抗凋亡的Bcl-2蛋白的作用,促进促凋亡蛋白Bax的表达,促进胰腺腺泡细胞的凋亡,减少胰腺腺泡细胞的坏死,减少炎症因子,如TNF-α与IL-6等的合成与释放。
AIM:To explore the effect of dexamethasone on the expression of Notch1,Hes1,Bax and Bcl-2 in pancreas in acute pancreatitis(AP).METHODS:Caerulein was used to set up AP animal model.The experimental groups were the saline group(control group),the model group(AP group)and dexamethasone group(DEX group).The content of TNF-αand IL-6 in serum in each group was detected using ELISA method.RT-qPCR was used to detect expression of Notch1,Hes1,Bax and Bcl-2 mRNA in pancreas in each group.Western blot was used to detect expression of Notch1,Hes1,Bax and Bcl-2 protein in pancreas in each group.RESULTS:The results of ELISA test showed that dexamethasone could significantly reduce the content of TNF-αand IL-6 in serum,comparing with the model group.The RT-qPCR and Western blot results showed that dexamethasone could significantly reduce the expression of Notch1,Hes1,Bcl-2 mRNA and protein in pancreas,but increase the expression of Bax mRNA and protein,as compared with the AP group.CONCLUSION:Dexamethasone could inhibit activation of the Notch signaling pathway,reduce expression of Notch1 and Hes1,further inhibit expression and antiapoptotic effect of Bcl-2 and promote expression and pro-apoptotic effect of Bax,promote apoptosis and reduce necrosis of pancreatic acinar cells,reduce the synthesis and release of TNF-αand IL-6.
作者
孔令宇
席錾
杨亚勤
马文婷
吴畏
郝同琴
KONG Lingyu;XI Zan;YANG Yaqin;MA Wenting;WU Wei;HAO Tongqin(Department of Emergency,The First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan,China;Department of Pediatric surgery,The First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan,China;Department of Diagnostic Laboratory of Sanquan College,Xinxiang Medical University,Xinxiang 453003,Henan,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2019年第2期134-139,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
2016年河南省组织再生重点实验室开放课题项目(KFKT16003)
