摘要
目的研究自噬相关基因微管相关蛋白1轻链3(LC3B)和雷帕霉素靶蛋白(mTOR)在吸烟与不吸烟口腔白斑(OLK)患者中的表达情况及相互关系,探讨吸烟与OLK恶变潜能的关系。方法采用免疫组织化学法检测LC3B和mTOR在120例OLK组织中的表达,回顾性分析吸烟者与不吸烟者的临床资料,探讨吸烟与LC3B和mTOR表达及其与各临床因素之间的关系。结果吸烟与不吸烟OLK患者在性别、年龄、病变部位、病理分型及恶变情况均存在差异,LC3B在不吸烟组中的阳性率高于相应的吸烟组,而mTOR在吸烟组中的阳性率则高于相应的不吸烟组,差异均有统计学意义(P<0.05),吸烟情况与mTOR阳性率呈正相关(P<0.05),与LC3B的表达无明显相关性,LC3B与mTOR阳性表达率随吸烟情况的加重呈负相关(P<0.05)。结论吸烟对OLK的影响可能与自噬相关蛋白的表达有关。
Objective This study aimed to investigate the expression patterns and relationship of microtubule-associated protein 1 light chain 3(LC3B)and mammalian target of rapamycin(mTOR)in oral leukoplakia(OLK)in smokers and neversmokers.This work also analyzed the relationship between smoking and the carcinogenic potential of OLK.Methods Immunohistochemistry was used to detect the expression of LC3B and mTOR in 120 patients with OLK.Clinical data from 120 smokers and never-smokers with OLK were analyzed.Subsequently,the relationships among LC3B and mTOR expression,clinical factors,and smoking were analyzed.Results Smoking and nonsmoking patients with OLK differed in terms of gender,age,lesion location,pathological typing,and carcinogenic situation.The positive rate of LC3B in never-smokers was higher than that in smokers.Whereas the positive rate of mTOR in smokers was higher than that in the corresponding never-smokers,and the differences were statistically significant(P<0.05).Smoking was positively correlated with the positive rate of mTOR(P<0.05),and had no significant correlation with LC3B expression.The positive rates of LC3B and mTOR were negatively correlated with the intensity of smoking(P<0.05).Conclusion The effect of smoking habits on OLK may be linked to the expression of proteins that are directly associated with autophagy.
作者
董小琳
柳志文
Dong Xiaolin;Liu Zhiwen(Stomatological Center,Xiangya Second Hospital of Central South University,Changsha 410011,China)
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2019年第1期19-24,共6页
West China Journal of Stomatology
关键词
微管相关蛋白1轻链3
雷帕霉素靶蛋白
吸烟
口腔
白斑
microtubule-associated protein 1 light chain 3
mammalian target of rapamycin
smoking
oral cavity
leukoplakia
