摘要
为提高产脲节杆菌DnL1-1的发酵水平,通过单因子筛选及正交试验,优化了DnL1-1的发酵培养基。优化得到的培养基配方为:葡萄糖15. 0 g/L,蛋白胨7. 5 g/L,酵母提取物0. 50 g/L,磷酸氢二钾2. 0 g/L,磷酸二氢钾1. 0 g/L,氯化钙0. 15 g/L,硫酸镁0. 15 g/L。配方在接种量为1%,180 r/min,28℃摇瓶振荡培养至36 h时,菌体数量达2. 89×1010cfu/m L以上。
In order to improve the biomass of Arthrobacter ureafaciens DnL1-1,the fermentation medium of the strain DnL1-1 was optimized by single factor screening and orthogonal experiment.The results showed that the best formula should be:glucose 15.0 g/L,peptone 7.5 g/L,yeast extract 0.50 g/L,K 2HPO 4 2.0 g/L,KH 2PO 4 1.0 g/L,CaCl 2 0.15 g/L,MgSO 4 0.15 g/L.Thus the strain can produce about 2.89×10^10 cells in 1 mL of the culture medium under the condition of 1%inoculum,with shaking of 180 r/min at 28℃for 36 hours.
作者
李红梅
魏艳丽
扈进冬
杨凯
刘宝军
杨合同
BAZHANAU Dzmitry
李纪顺
LI Hong-mei;WEI Yan-li;HU Jin-dong;YANG Kai;LIU Bao-jun;YANG He-tong;BAZHANAU Dzmitry;LI Ji-shun(Shandong Provincial Key Laboratory of Applied Microbiology,Ecology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China)
出处
《山东科学》
CAS
2019年第1期52-56,共5页
Shandong Science
基金
国家重点研发计划(2017YFD0201706)
山东省博士基金(ZR2017BC075)
山东省科学院青年基金(2018QN0020
2018QN0021)
山东省科学院国际合作项目(2019DH2D11)
关键词
产脲节杆菌
阿特拉津
发酵培养基
正交设计
Arthrobacter ureafaciens
atrazine
fermentation medium
orthogonal design
