摘要
以崂山绿茶为原料,分别用水、石油醚、氯仿、乙酸乙酯、正丁醇进行脂肪酶抑制剂活性部位筛选,运用超声辅助提取的方法,考察研究乙醇浓度、超声波功率、超声提取温度和超声提取时间对崂山绿茶中脂肪酶抑制剂的得率和抑制率的影响,通过正交试验得到超声波辅助法提取崂山绿茶中脂肪酶抑制剂的最佳工艺条件。结果表明:崂山绿茶中水、石油醚、氯仿、乙酸乙酯、正丁醇组分对脂肪酶抑制的IC_(50)值分别为3.95、9.64、34.73、0.69、21.61 mg/mL;较佳的工艺条件为:300 W功率下,用浓度为60%的乙醇,在30℃下浸提10 min,用乙酸乙酯萃取后浓缩并冷冻干燥提取物,最终提取物得率为29.2%,对脂肪酶抑制率为79.2%。
Taking Laoshan green tea as raw material,the active parts of lipase inhibitors were screened by water,petroleum ether,chloroform,ethyl acetate and n-butanol,and the extraction rate of ethanol concentra-tion,ultrasonic power,ultrasonic extraction temperature and ultrasonic extraction time on the yield of lipase inhibitors in Laoshan green tea was investigated by ultrasonic assisted extraction.The best conditions of ultrasonic extraction of lipase inhibitor from Laoshan green tea were obtained by orthogonal test.The results showed that the IC50 values of water,petroleum ether,chloroform,ethyl acetate and n-butanol in Laoshan green tea were 3.95,9.64,34.73,0.69 mg/mL and 21.61 mg/mL,respectively.The better technological conditions were:300 W power,60%ethanol concentration,10 minutes at 30℃and ethyl acetate extraction.After extraction,the extract was concentrated and freeze-dried.The final extraction yield was 29.2%,and the inhibition rate on lipase was 79.2%.
作者
任秀娟
REN Xiu-juan(Food Engineering Department,Shandong Business Institute,Yantai 264670,Shandong,China;Research&Development Center of Food Engineering Technology,Shandong Business Institute,Yantai 264670,Shandong,China)
出处
《食品研究与开发》
CAS
北大核心
2019年第5期71-75,共5页
Food Research and Development
基金
山东商务职业学院2017年院级科研基金重点课题项目(2017SWZR01)
关键词
崂山绿茶
脂肪酶抑制剂
活性筛选
超声辅助提取
提取工艺
Laoshan green tea
lipases inhibitor
activities screening
ultrasonic-assistant extraction
extracting process
