摘要
以自制3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂P(GMA/EDMA)为载体,间氨基苯硼酸为配体制备了一种新型高效硼酸基亲合色谱填料.考察了抗生素:去甲万古霉素、阿米卡星、异帕米星和庆大霉素,与硼酸配体的特异性作用,以及流动相中盐离子浓度和pH对抗生素保留的影响.结果表明:表面带有邻羟基的抗生素和硼酸基具有特异性亲合作用,且抗体结构中具有不同的邻羟基数目表现出不同的保留能力.用所合成的硼酸基亲合色谱柱纯化去甲万古霉素,结合反相色谱检测其纯化液,证明所制备的硼酸基亲和色谱柱具备纯化去甲万古霉的能力.
A novel boric acid-affinity chromatographic packings was prepared by using 3.0 μm non-porous monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads (P(GMA/EDMA) as matrix and M-aminophenylboric acid (APBA) as ligand.The specific effects of antibiotics, such as norvancomycin, amikacin, isopamicin and gentamicin, with boric acid ligands, as well as the effects of salt ion concentration and pH in mobile phase on the retention of antibiotics were investigated.The results indicated that antibiotics with o- hydroxyl groups and borate groups had affinity specificity, and antibiotics with the different number of o- hydroxyl groups in structure showed different retention ability.Furthermore, the norvancomycin was purified by boric acid affinity column and the purified solution was determined by reversed-phase column, proving that the developed boric acid affinity chromatographic column in this study was capable of purifying norvancomycin.
作者
卜春苗
龚波林
陈卫民
BU Chunmiao;GONG Bolin;CHEN Weimin(College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China;Institute of Environmental Engineering,Ningxia University,Yinchuan 750021,China;College of Chemistry and Chemical Engineering,North Universities for Nationalities,Yinchuan 750021,China)
出处
《信阳师范学院学报（自然科学版）》
CAS
北大核心
2019年第1期132-136,共5页
Journal of Xinyang Normal University(Natural Science Edition)
基金
国家自然科学基金项目(21266024)
宁夏大学科学研究基金项目(ZR1711)
关键词
聚甲基丙烯酸环氧丙酯树脂
硼酸
亲合色谱
抗生素
poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads
boric acid
affinity chromatography
antibiotic
