摘要
为探讨玉米对锰胁迫响应机制,研究不同基因型玉米垦粘1号(K)、双玉201(S)、东青1号(D)、吉农大516(J)和先玉335(X)在不同锰浓度0 mmol·L^(-1)(Mn1)、0.005 mmol·L^(-1)(CK),5 mmol·L^(-1)(Mn2),10 mmol·L^(-1)(Mn3)、20 mmol·L^(-1)(Mn4)下,植株株高、根长、生物量、锰含量、玉米抗氧化酶活性[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)]及丙二醛含量(MDA)的影响。结果表明,锰胁迫抑制植株生长发育。玉米株高较CK下降2.83～18.13 cm;在Mn4条件下根长较CK下降200.27～434.77 cm,根表面积下降14.82～132.85 cm^2。生物量也呈下降趋势,地上部生物量受抑制程度大于根部。J基因型变化程度较小,S基因型变化较大。随锰胁迫增强,D基因型SOD、POD、CAT活性随锰浓度增加而增加,其他基因型均有不同程度下降,MDA含量D基因型随锰浓度变化较平缓,S基因型增幅较大,D基因型抗性较强,S基因型抗性较弱。
In order to reveal the response mechanism of maize to manganese stress,different maize genotypes,Kennian1(K),Shuangyu201(S),Dongqing1(D),Jinongda516(J)and Xianyu335(X),were studied at different manganese concentrations with0mmol·L^-1(Mn1),0.005mmol·L^-1(CK),5mmol·L^-1(Mn2),10mmol·L^-1(Mn3),20mmol·L^-1(Mn4).The plant height,root length,biomass,manganese content,activities of antioxidant enzymes(SOD,CAT,POD)and MDA content in maize were measured.The results showed that manganese stress inhibited plant growth.Compared with0.005mmol·L^-1(CK),maize plant height decreased by2.83-18.13cm,while the root length decreased by200.27-434.77cm and the root surface area decreased by14.82-132.85cm2under Mn4treatment.The overground part biomass was inhibited more than the root biomass.The change of J genotype was small,while that of S genotype was large.With the increase of manganese stress,the activities of SOD,POD and CAT of maize D genotype were increased,while the activities of other maize genotypes were decreased.The changes of MDA content in D genotype with manganese concentration increasing were gentle;the increase of S genotype was larger than other genotypes.The resistance of D genotype was stronger than S genotype.
作者
张迪
吕思琪
张婉婷
姜佰文
ZHANG Di;LV Siqi;ZHANG Wanting;JIANG Baiwen(School of Resources and Environmental Sciences,Northeast Agricultural University, Harbin 150030, China;Heilongjiang Fertilizer Engineering Research Center, Harbin 150086, China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2018年第12期27-35,共9页
Journal of Northeast Agricultural University
基金
国家科技支撑计划项目(2017YFD0300503)
关键词
玉米
基因型
锰
抗氧化酶
丙二醛
maize
genotype
manganese
antioxidases
malondialdehyde
