摘要
目的研究茄病镰刀菌感染树鼩角膜过程中甘露糖受体和炎症因子的表达变化,探讨真菌感染性角膜炎发生机制。方法通过接触镜辅助法用茄病镰刀菌感染树鼩角膜,前段照相、病理切片及活体共聚焦显微镜观察结果对动物模型进行评价。qPCR检测树鼩角膜炎病发过程中的甘露糖受体、dectin-1、IL-1β、IL-6、TNF-α的表达;体外培养树鼩原代角膜细胞并进行感染实验。结果树鼩真菌性角膜炎模型建立成功率为84%。MR与dectin-1在感染初期有高拷贝数阳性表达,引起炎症因子IL-1β、IL-6、TNF-α的表达峰值,这些因子的表达与病症的严重程度呈正相关。角膜细胞体外感染前后IL-1β、IL-6、TNF-α的表达差异无显著性(P> 0.05)。结论建立的真菌性角膜炎树鼩模型,其病理表现相与人类真菌性角膜炎症状接近。甘露糖受体可能参与调控机体对真菌感染的免疫应答反应,机体自身的免疫能力可能对角膜炎病发与转归有重要作用。
Objective To determine the changes in the expression of mannose receptor and some inflammatory cytokines in the cornea of tree shrews infected by Fusarium solanum,and explore the mechanism of fungal infection keratitis.Methods A tree shrew cornea was infected by Fusarium solanum using a contact lens-assisted method,The animal model was evaluated by anterior segment photography,analysis of pathological sections,and in vivo confocal microscopy.qPCR was used to determine the expression of MR(mannose receptor),dectin-1,IL-1β,IL-6,and TNF-αin the tree shrew keratitis.The primary corneal cells of tree shrews were cultured and an infection experiment was carried out in vitro.Results The success rate of establishing the tree shrew fungal keratitis model was84%.The positive expression of MR and dectin-1at the early stage of infection caused peaks in the expression of IL-1β,IL-6,and TNF-α;the expression of these factors was positively correlated with the severity of fungal keratitis in the tree shrews.There was no significant change in corneal epithelial cell infection before and after infection(P>0.05).Conclusions The model of fungal keratitis in tree shrew is successfully established,and its symptoms are similar to those of human fungal keratitis.Type C lectin receptors may be involved in regulating the immune response to fungal infection.The immune system may play an important role in the development and progression of keratitis.
作者
苗雨润
贾杰
黎晓慧
袁园
宋庆凯
张志成
李娜
代解杰
MIAO Yurun;JIA Jie;LI Xiaohui;YUAN Yuan;SONG Qingkai;ZHANG Zhicheng;LI Na;DAI Jiejie(Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, the Chinese Academy of Medical Scienceand Peking Union Medical College. Yunnan Innovation Team of Standardization and Application Research of TreeShrew. the Key Laboratory of Yunnan Province for Ophthalmic Research and Disease Control. Kunming 650118, China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2018年第6期752-759,共8页
Acta Laboratorium Animalis Scientia Sinica
基金
云南省联合支持国家计划项目(2015GA009)
云南省科技人才和平台计划项目(2017HC019)
重点实验室运行补助专项(2017DG008).
