摘要
试验旨在研究不同品系大白猪骨形态发生蛋白受体ⅠB(bone morphogenetic protein receptor-ⅠB,BMPR-ⅠB)基因多态性及其与繁殖性状的相关性。采用Hi-SNP中高通量基因分型技术对BMPR-ⅠB基因多态位点进行分型,利用SPSS统计软件,采用最小二乘法将各位点不同基因型与总产仔数、产活仔数、死胎数、畸形头数进行相关性分析。结果显示,不同品系大白猪在BMPR-ⅠB基因746A>G和852G>A位点均未检测到多态性,804G>C位点上均表现为GG基因型为优势基因型,G为优势等位基因;960C>T位点在美系大白猪中以CC基因型频率较高,而在英系大白猪中以TT基因型频率较高,在加系大白猪中CT基因型频率较高,3个不同品系大白猪群体在804G>C和960C>T位点均处于Hardy-Weinberg平衡状态(P>0.05)。804G>C位点在3个不同品系大白猪中对总产仔数、产活仔数、死胎数、畸形数等繁殖性状均无显著影响(P>0.05)。960C>T位点在美系大白猪群体中TT基因型死胎数极显著低于CC基因型1.027头(P<0.01);英系大白猪群体中TT基因型总产仔数显著高于CC基因型1.976头(P<0.05),极显著高于CT基因型2.118头(P<0.01);加系大白猪中TT基因型产活仔数显著高于CC基因型0.886头(P<0.05),死胎数、畸形头数显著低于CC基因型0.707和0.337头(P<0.05)。960C>T位点中TT基因型可作为3个不同品系大白猪繁殖性状的标记基因型。
This study was aimed to investigate the polymorphism of bone morphogenetic protein receptor-ⅠB(BMPR-ⅠB)gene and its correlation with the reproductive traits in Large White pigs.The polymorphism of BMPR-ⅠB gene was analyzed by high-throughput sequencing technologiy,the association of different genotypes of BMPR-ⅠB gene with the total number of births,alive births,deaths and deformities using the least square method by SPSS statistical software.The results showed that the polymorphism was not detected in BMPR-ⅠB gene 746A>G and 852G>A loci,in the 804G>C locus,the frequency of GG genotype was dominant genotype,and G was the dominant allele;In the 960C>T locus,the frequency of genotypes CC,TT and CT were higher in American,British and Canadian Large White pigs,respectively.The results ofχ2 test showed that the 804G>C and 960C>T loci were fit with Hardy-Weinberg equilibrium in the different Large White pigs lines(P>0.05).There were no significant differences in the effects of 804G>C locus on the number of total births,alive births,still births,deformity and other reproductive traits of the three different lines of Large White pigs(P>0.05).The number of stillbirths with TT genotype in American Large White pigs were significantly lower than that of CC genotype in 960C>T locus(P<0.05),which was decreased by 1.027;The total litter size of the British Large White pigs with TT genotype was significantly higher than that of CC genotype(P<0.05),and extremely significantly higher than that of CT genotype(P<0.01),which was in-creased 1.976 and 2.118,respectively;The number of live births in Canadian Large White pigs with TT genotype was significantly higher than that of CC genotype(P<0.05),the stillbirth and deformity of TT genotype were significantly lower than that of CC genotype(P<0.05),which were decreased 0.707 and 0.337,respectively.The TT genotype in 960C>T locus could be used as the marker genotype for reproductive traits of three different lines of Large White pigs.
作者
胡慧艳
贾青
侯胜奎
刘津
刘洋洋
张伟峰
赵坤峰
王贵江
HU Huiyan;JIA Qing;HOU Shengkui;LIU Jin;LIU Yangyang;ZHANG Weifeng;ZHAO Kunfeng;WANG Guijiang(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China;National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071000,China;Hebei Shunde Tianzhao Livestock Technology Co.,Ltd.,Wanquan 076250,China;School of Life Science and Food Engineering,Hebei University of Engineering,Handan 056000,China;Hebei Dongwang Breeding Co.,Ltd.,Ningjin 055550,China;Animal Husbandry and Breeding Station of Hebei,Shijiazhuang 050061,China)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第4期984-991,共8页
China Animal Husbandry & Veterinary Medicine
基金
河北省研究生创新资助项目(CXZZBS2017068)
河北省现代农业产业技术体系专项(HBCT2013070202)
