摘要
目的探讨虎杖对慢性阻塞性肺病(COPD)模型大鼠肺组织基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶抑制剂-1(TIMP-1)表达的影响。方法 SD雄性大鼠40只,随机分成正常对照组、COPD模型组、强的松组(6mg/kg)、虎杖组(6g/kg),各10只,相应药物干预28天。选用熏烟和气管内注入脂多糖(LPS)法建立大鼠COPD模型。Real-Time PCR方法检测肺组织MMP-9和TIMP-1表达。结果与正常对照组[(0.47±0.04)、(0.46±0.04)]比较,COPD模型组[(0.92±0.06)、(1.06±0.03)]、强的松组[(0.60±0.05)、(0.63±0.05)]、虎杖组[(0.86±0.04)、(0.95±0.04)]大鼠MMP-9和TIMP-1表达水平均明显升高(P<0.05);与COPD模型组相比,强的松组、虎杖组大鼠MMP-9和TIMP-1表达水平均显著降低(P<0.05)。结论虎杖可降低COPD模型大鼠MMP-9和TIMP-1表达,对肺组织有一定保护作用,这可能与其控制气道炎症及抑制重塑的作用有关。
Objective To investigate the effects of Polygoni cuspidata on the expression of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in lung tissue of chronic obstructive pulmonary disease(COPD)rats.Methods COPD model was established by fumigation and intratracheal injection of lipopolysaccharide(LPS).Forty male SD rats were randomly divided into 4 groups with 10 rats in each group:control group,COPD model group,prednisone group and Polygonum cuspidatum intervention group.0.5h before each fumigation,the first 29 days of fumigation with Polygonum cuspidatum decoction,prednisone-related intervention for 28 days.On the 28th day of administration,the rats were sacrificed.Real-Time PCR method was used to detect the expression of MMP-9 and TIMP-1 in lung homogenates.Results The expression levels of MMP-9(0.47±0.04)and TIMP-1(0.46±0.04)in the control group were lower than those in COPD model group(0.92±0.06,1.06±0.03),prednisone group(0.60±0.05,0.63±0.05)and Polygonum cuspidatum intervention group(0.86±0.04,0.95±0.04),with all P<0.05.Compared with the COPD model group,the expression levels of both index in the prednisone group and the Polygonum cuspidatum group were significant decreased(P<0.05).Conclusion Polygonum cuspidatum can decrease the expression of MMP-9 and TIMP-1 and protect the lung tissue of COPD rats,which may be related to the control of airway inflammation and inhibition of remodeling.
作者
石亚莉
楼黎明
陈素珍
王世强
胡丹丹
黄冉冉
徐一凯
SHI Yali;LOU Liming;CHEN Suzhen;WANG Shiqiang;HU Dandan;HUANG Ranran;XU Yikai(The 3rd College of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou(310053),China;Department of Respiratory Diseases,the 3rd Hospital Affiliated to Zhejiang Chinese Medical University,Hangzhou(310005),China)
出处
《浙江中西医结合杂志》
2018年第4期271-273,281,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江中医药大学附属第三医院院级医药卫生科技计划(No.ZS16ZA01)
浙江中医药大学校级科研基金(No.2013ZR03)资助
关键词
大鼠
慢性阻塞性肺病
虎杖
肺组织
基质金属蛋白酶-9
金属蛋白酶抑制剂-1
rats
chronic obstructive pulmonary disease
Polygonum cuspidatum
lung tissue
matrix metalloproteinase-9
matrix protein metalloproteinase-1
