摘要
采用菌丝生长速率法测定了不同条件下白僵菌菌株S1-7发酵液对桉树枝枯病菌可可毛色二孢(Lasiodiplodiatheobromae)抑制作用的影响。结果表明,抑菌率最高的培养条件:温度为28℃,培养时间20 d,p H值7.0~8.0,培养基中葡萄糖、蛋白胨、酵母粉含量分别为40、20、20 g/L。此外,60℃以下高温处理发酵液4 h对抑制作用影响不大,温度高于60℃抑菌率显著降低;紫外线照射对发酵液的抑菌率影响不大。
Inhibitory effects ofBeauveria bassiana strain S1-7 on pathogens Lasiodiplodia theobromae causing die back of Eucalyptus spp.were determined under different conditions by mycelium growth rate method.Results showed that the inhibition rate was the highest when culture temperature was 28℃,the incubation time was 20 d,the pH value was from 7.0 to 8.0,and medium glucose,protein peptone and yeast powder content were 40 g/L,20 g/L and 20 g/L,respectively.In addition,the fermentation broth had little influence on inhibitory effect when the high temperature was under 60℃for 4 hours,but inhibition rate decreased significantly when the temperature was higher than 60℃.The UV irradiation had little influence on the inhibition rate of the fermentation broth.
作者
邹东霞
廖旺姣
黄华艳
蒋学建
ZOU Dong-xia;LIAO Wang-jiao;HUANG Hua-yan;JIANG Xue-jian(Guangxi Forestry Research Institute,Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning 530002,China)
出处
《广西林业科学》
2018年第1期85-88,共4页
Guangxi Forestry Science
基金
广西林科院基本科研业务费专项(林科201610号)
关键词
白僵菌
发酵液
可可毛色二孢
抑制作用
Beauveria bassiana
fermentation broth
Lasiodiplodia theobromae
inhibitory effect
