摘要
目的考察安石榴苷在不同pH值磷酸盐缓冲液中的平衡溶解度(S)和油水分配系数(PO/W),为其制剂开发提供参考。方法采用摇瓶-紫外分光光度法测定安石榴苷在不同温度(25℃、37℃)、pH为1.2、2.5、5.0、5.8、6.8、7.4、7.8~8.0的磷酸盐缓冲液中的S值和在正辛醇-缓冲液中的PO/W值。结果在pH为1.2~8.0条件下,安石榴苷在25℃时的S值和lgPO/W分别为775.00~2 789.00mg/mL和-3.07^-3.85,在37℃时的S值和lgPO/W分别为1 006.00~2 684.00mg/mL和-1.32^-3.81;在pH 1.2~8.0范围,均有lgPO/W<0,pH为1.2时,安石榴苷的S值较低。结论安石榴苷水溶性较好,亲脂性差,在胃内的溶解性不好,表明透膜性差是影响胃肠道吸收的限速因素。
ObjectiveTo determine the equilibrium solubility(S)and the apparent oil/water partition coefficient(PO/W)of punicalagin in different pH phosphate buffer solution,so as to provide the basis for the exploitation of preparations.MethodsThe S values and the PO/W values of punicalagin in pH1.2,2.5,5.0,5.8,6.8,7.4,7.8-8.0 of phosphate buffer solutions at 25℃or 37℃were measured by shaking flask-UV spectrophotometry.ResultsIn pH 1.2-8.0 phosphate buffer solutions,the S values of punicalagin were 775.00~2 789.00 mg/mL and the PO/W values were-3.07^-3.85 at 25℃,and the S values of punicalagin was 1 006.00~2 684.00 mg/mL and the PO/W values were-1.32^-3.81at 37℃.The lgPO/W values were<0 at the pH range from 1.2 to 8.0.The S value of punicalagin in pH1.2 is markedly lower than the others.ConclusionPunicalagin has good watersolubility,but its solubility is bad in stomach.Punicalagin has poor liposolubility.It may be poor absorption in the gastrointestinal tract for the poor membrane permeability.
作者
张慧慧
施洋
张晓霞
马晓丽
田莉
ZHANG Huihui;Shi Yang;ZHANG Xiaoxia;MA Xiaoli;TIAN Li(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830011,China;Department of Journal Editorial,Xinjiang Medical University,Urumqi 830011,China;Center of Instrumental Analysis,Xinjiang Medical University,Urumqi 830011,China)
出处
《新疆医科大学学报》
CAS
2018年第2期225-228,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金(81260668)
乌鲁木齐市科技创新项目(G161320011)
关键词
安石榴苷
平衡溶解度
油水分配系数
punicalagin
equilibrium solubility
apparent oil-water partition coefficient
