摘要
建立测定茶叶中23种有机磷农药残留量的气相色谱检测方法。茶叶样品经丙酮提取后,采用Qu ECh ERS方法前处理,以N-丙基乙二胺(N-propyl ethylenediamine,PSA)、多壁碳纳米管(multi walled carbon nanotubes,MWCNTs)吸附提取液中的干扰组分,净化液经气相色谱测定,基质外标法定量。采用本实验建立的方法,在0.02~1.00μg/m L范围内,23种有机磷的线性关系良好,相关系数r大于0.986 5,方法检出限为0.01~0.02 mg/kg,定量限为0.03~0.06 mg/kg。在0.05、0.50、1.00 mg/kg三个添加量的平均加标回收率为81.8%~107.2%,相对标准偏差为1.2%~7.2%。与传统的Qu ECh ERS相比,该方法具有操作简单、快速、试剂用量少、回收率高、准确性强等特点,适合于茶叶中23种有机磷农药残留量的测定。
This study intended to develop a method for the determination of23organophosphorus pesticide residues in tea by gas chromatography(GC).The target pesticides in tea were extracted by a modified QuEChERS method with acetone as the solvent,followed by cleanup with N-propyl ethylenediamine(PSA)adsorbent and multi-walled carbon nanotubes(MWCNTs).The quantification was performed by the matrix external standard method.Good linearity was observed for all calibration curves with correlation coefficients(r)>0.9865over the range of0.02–1.00μg/mL.The limits of detections(LODs)were0.01–0.02mg/kg,and the limits of quantitation(LOQs)were0.03–0.06mg/kg.The recoveries at three spiked levels(0.05,0.50and1.00mg/kg)varied from81.8%to107.2%,with relative standard deviations(RSDs)of1.2%–7.2%.Compared with the traditional QuEChERS method,this method was easy,fast and solvent saving with good recovery and accuracy.Thus,it was suitable for the determination of23organophosphorus pesticide residues in tea.
作者
黄田田
汤桦
董晓倩
刘松南
许雯雯
王华
宗琦
HUANG Tiantian;TANG Hua;DONG Xiaoqian;LIU Songnan;XU Wenwen;WANG Hua;ZONG Qi(Beijing Center for Tea Qualify Supervision and Inspection, Beijing Academy of Food Sciences, Beijing 100162, China;National Institute of Metrology, Beijing 100029, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第6期315-321,共7页
Food Science
