摘要
目的:研究血清可溶性E-钙黏素(sE-cadherin)、趋化因子配体16(CXCL16)含量对宫颈癌病灶内恶性生物学行为的评估价值。方法:选择2014年8月~2017年4月期间在黄冈市中心医院接受手术治疗的宫颈癌患者作为研究的宫颈癌组,选择同期在黄冈市中心医院体检的健康者作为对照组。测定宫颈癌组和对照组血清中sE-cadherin、CXCL16的含量以及宫颈癌组患者宫颈癌病灶、癌旁病灶内增殖基因、侵袭基因的mRNA表达量。结果:宫颈癌组患者血清中sE-cadherin的含量显著低于对照组,CXCL16的含量显著高于对照组;宫颈癌病灶组织中SP2、SND1、ANGPTL4、S6K1、Rab11、S100A6、N-cadherin、MMP9的mRNA表达量显著高于癌旁病灶组织,且与血清中sE-cadherin的含量呈负相关、与血清中CXCL16的含量呈正相关,TET1、HSG、FHIT的mRNA表达量显著低于癌旁病灶组织,且与血清中sE-cadherin的含量呈正相关、与血清中CXCL16的含量呈负相关。结论:宫颈癌患者血清中降低的sE-cadherin以及升高的CXCL16能够评估病灶内增殖及侵袭的恶性生物学行为。
Objective:To study the value of serum sE-cadherin and CXCL16contents for the evaluation of malignant biological behaviors in cervical cancer.Methods:Patients with cervical cancer who accepted surgical treatment in Huanggang Central Hospital between August2014and April2017were selected as the cervical cancer group of the research and healthy subjects who received physical examination in Huanggang Central Hospital over the same period were selected as the control group.The contents of sE-cadherin and CXCL16in the serum of cervical cancer group and control group as well as the mRNA expression of proliferation genes and invasion genes in cervical cancer lesion and adjacent lesion of cervical cancer group were determined.Results:Serum sE-cadherin content of cervical cancer group was greatly lower than that of control group whereas CXCL16content was greatly higher than that of control group;SP2,SND1,ANGPTL4,S6K1,Rab11,S100A6,N-cadherin and MMP9mRNA expression in cervical cancer lesion were greatly higher than those in adjacent lesion,negatively correlated with serum sE-cadherin content,and positively correlated with serum CXCL16content whereas TET1,HSG and FHIT mRNA expression were greatly lower than those in adjacent lesion,positively correlated with serum sE-cadherin content,and negatively correlated with serum CXCL16content.Conclusion:The decreased sE-cadherin and increased CXCL16in serum of patients with cervical cancer can assess the malignant biological behaviors of proliferation and invasion in the lesion.
作者
肖欢
罗大极
XIAO Huan;LUO Da-ji(School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, 430077;Gynecology Department, Huanggang Central Hospital in Hubei Province, Huanggang, Hubei Province, 438000)
出处
《海南医学院学报》
CAS
2017年第24期3433-3436,共4页
Journal of Hainan Medical University
基金
国家自然科学基金(30900853)~~
