摘要
采用茎段作为外植体,建立独脚金Striga asiatica的组织培养快速繁殖方法。结果显示,0.1%升汞溶液处理7~9 min是较有效的消毒方法;诱导不定芽增殖培养基适宜配方为MS+6-BA 3.0 mg·L^(-1)+IBA 0.1mg·L^(-1);生根培养基配方为MS+NAA 0.1 mg·L^(-1)+IBA 0.5 mg·L^(-1),培育周期为3个月。
Stems of Striga asiatica were used as the explants to establish optimum conditions for rapid propagation in vitro.The results showed that disinfecting the stems7-9min with0.1%mercuric chloride solution was the best methods;MS medium with3.0mg·L-16-BA and0.1mg·L-1IBA was optimum for inducing adventitious and proliferation reproduction.The suitable medium for rooting was MS+0.1mg·L-1NAA+0.5mg·L-1IBA.
作者
蔡时可
魏杰
李战超
王继华
CAI Shi-ke;WEI Jie;LI Zhan-chao;WANG Ji-hua(Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong China;College of Life Sciences, South China Agricultural University,Guangzhou 510642, Guangdong China)
出处
《亚热带植物科学》
2017年第4期375-378,共4页
Subtropical Plant Science
关键词
独脚金
组织培养
快速繁殖
Striga asiatica
tissue culture
rapid propagation
