摘要
分别用Sub G1法和PA法对人类早幼粒细胞急性白血病细胞株 (HL 6 0 )的自发凋亡进行检测 ,发现HL 6 0细胞自发凋亡时 ,在DNA直方图上 ,G1峰前出现亚G1峰 (Sub G1峰 ) ,G1期细胞增加 ,S期和G2 /M期细胞减少。同时G1期细胞发生自发凋亡 ,而S期 ,G1期细胞少有凋亡发生。
Apoptotic cells were analyzed with Sub G1 and PA methods. Apoptotic cells were distinguished by Sub G1 on DNA histogram. Late apoptotic cells can be detected simply by Sub G1. The cell cycle specificity of apoptosis can be analyzed by PA.The cell cycle specificity of apoptosis of cancer cell can also be analyzed in a simple yet efficient way.
出处
《武汉科技大学学报》
CAS
2002年第3期308-309,共2页
Journal of Wuhan University of Science and Technology
基金
国家自然科学基金资助项目 (3 973 0 2 70 )
国家杰出青年基金资助项目(3 972 5 0 2 7)
973肿瘤计划项目(H19980 5 12 12 )
卫生部临床重点学科资助 (970 70 2 3 9)项目
