摘要
为了建立应用于神经组织工程的感觉神经和运动神经的 Schwann细胞的体外培养方法 ,采用联合酶消化法对 SD乳鼠后根神经和股神经运动支的 Schwann细胞进行了培养 ,并经抗 S10 0荧光染色鉴定和双抗体夹心间接 Elisa法测量两种 Schwann细胞培养基中 NGF的表达。结果表明 ,两种培养细胞经荧光染色证明均为 Schwann细胞 ,细胞纯度均超过 95 % ,光镜观察未见形态学差异 ,但 NGF的表达量和表达模式均有显著性差别 (P<0 .0 5 )。提示 ,本实验方法可以获得高纯度的感觉神经和运动神经的 Schwann细胞 ,两者的生物学功能有一定差异。
To establish the culture method of Schwann cells from sensory and motor nerves for the use of nerve tissue engineering, sensory and motor Schwann cells were cultured respectively from dorsal root nerves and femoral nerve's motor rami of 5 day old SD rats. The cultured Schwann cells were identified by anti S 100 immunofluorescent staining, and the NGF expression of two kind of cells in the culture media was measured by Sandwich Elisa. Both kinds of cells were proved to be Schwann cells. No obvious difference was observed by photomicrograph, but their NGF expression was different in both quantity and pattern (P<0.05). The present results indicate that high purity of sensory and motor Schwann cells needed by nerve tissue engineering can be prepared by this method, and there is some biological function difference between them. (Figures 1～6 on plate 46)
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2002年第3期219-222,T004,共5页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金 (No. 3 960 0 14 9)资助项目
