摘要
为评价梅州离子型稀土矿废弃地不同土壤改良措施对大豆根瘤菌多样性的影响,通过nif H-PCR-DGGE技术对不同土壤改良处理条件下大豆根瘤菌群落的多样性进行表征。结果表明:7种不同土壤改良处理的DGGE图谱条带数目不等,位置各异,说明梅州离子型稀土矿废弃地大豆的根瘤菌群落结构受改良措施的影响较大,具有较强的可塑造性。其中T4处理(有机肥+生物炭+钝化剂)的条带数最多,多样性指数最高,说明该处理对增加大豆根瘤菌的多样性效果最好,可作为以后土壤改良的参考依据。聚类分析结果显示:7个处理可分为4个类群,CK单独为一个类群,T1(有机肥)、T2(有机肥+生物炭)为一个类群,T4(有机肥+生物炭+钝化剂)和T5(有机肥+菌剂+蚯蚓)为一个类群,T3(有机肥+钝化剂)和T6(有机肥+菌剂+蚯蚓+生物炭+钝化剂)为一个类群。生物炭与钝化剂组合处理、菌剂与蚯蚓的组合处理对增加大豆根瘤内根瘤菌的多样性效果较显著。通过序列比对和系统发育树可以看出,在梅州黄畲地区稀土采矿迹地,大豆主要与慢生根瘤菌属菌株共生结瘤,也可与中华根瘤菌属菌株共生。大豆根瘤内根瘤菌的多样性与土壤镉含量之间为显著正相关关系,与土壤有机质、氮、磷等营养指标之间为负相关关系,说明根瘤菌可能在提高大豆耐镉方面具有重要作用。
In order to evaluate the effect of different improvement measures on the diversity of rhizobium of soybean(Glycinemax) in the abandoned land of ionic rare earth mine,nif H-PCR-DGGE was used to manifestate the diversity of Rhizobia. Theresults of denaturing gradient gel electrophoresis(DGGE) fingerprint showed that there were differences in the number,thepositions and the brightness of bands depended on different restorative treatments,which showed that different soil amendmentshad a significant effect on the community structure of Rhizobium. The number and diversity index of bands of T4 treatment(fertilizer + biochar + deactivator) were highest,it was best to improve the diversity of rhizobium of soybean. The results ofcluster analysis showed that 7 treatments could be divided into four groups,CK was group 1,T1 and T2 were group 2,T4 andT5 were group 3,T3 and T6 were group 4. The combination of biochar and passivator,the combination of bacterial manureand earthworm had the more significant effect on increasing the diversity of rhizobia in root nodules of soybean. According tosequence comparison and phylogenetic tree,soybean is mainly lived in symbiosis with Bradyrhizobium,and also coexisted withSinorhizobium in the abandoned land of ionic rare earth mine of Meizhou,Guangdong. There was significant positive correla-tion between the diversity of rhizobium and soil cadmium content,and negative correlation among the diversity of rhizobiumand soil organic matter,nitrogen,phosphorus and other nutritional indicators,which suggesting that rhizobium may play animportant role in improving the cadmium tolerance of soybean.
作者
刘明骞
张新鑫
马佳慧
李其斌
陈丽君
周玮
马玲
陈晓阳
LIU Ming-qian;ZHANG Xin-xin;MA Jia-hui;LI Qi-bin;CHEN Li-jun;ZHOU Wei;MA Ling;CHEN Xiao-yang(Preparation Office of South China Agricultural Museum,South China Agricultural University,Guangzhou 510642,China;Guangdong Province Research Center of Woody Forage Engineering Technology,South China Agricultural University,Guangzhou 510642,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China;Center for Teaching&Research Base,South China Agricultural University,Guangzhou 510642,China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,South China Agricultural University,Guangzhou 510642,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2019年第1期77-83,共7页
Soybean Science
基金
中央财政林业示范推广项目[(2015)GDTK-08]
广东省林业科技创新项目(2017KJCX033)
广东省科技计划(2015A020209139
2015B020207002)
广东省大学生创新训练项目(201710564034)
关键词
大豆
离子型稀土矿
根瘤菌
DGGE
多样性
Soybean(Glycine max)
Ionic rare earth mine
Rhizobia
DGGE
Diversity
