摘要
为探究2种稻飞虱——褐飞虱Nilaparvata lugens(St?l)和白背飞虱Sogatella furcifera(Horváth)诱导的水稻防御反应差异,于室内测定了水稻在分别受褐飞虱或白背飞虱产卵雌成虫为害时,其茉莉酸、水杨酸、乙烯、H_2O_2以及挥发物含量的变化。结果表明,尽管褐飞虱和白背飞虱产卵雌成虫的为害均可以诱导水稻茉莉酸、水杨酸、乙烯和H_2O_2等防御相关信号分子以及一些水稻挥发物含量的增加,但是二者的诱导作用存在差异。水稻在受白背飞虱产卵雌成虫为害时,茉莉酸的含量在3 h时就显著升高,12 h时含量达到最高;而受褐飞虱产卵雌成虫为害时,6 h时茉莉酸含量才显著升高,72 h时含量达最高;并且在2种稻飞虱为害的3～48 h内,白背飞虱为害诱导的茉莉酸含量始终显著高于褐飞虱为害诱导的。水稻受白背飞虱产卵雌成虫为害24 h后诱导的水杨酸含量、为害48 h后诱导的乙烯含量、为害72 h后诱导的H_2O_2含量及为害24 h后诱导的挥发物释放量分别是褐飞虱产卵雌成虫为害诱导的1.28、1.45、4.10和1.77倍。表明水稻能识别褐飞虱和白背飞虱的为害,从而做出针对害虫种类特异性的防御反应;并且水稻对白背飞虱产卵雌成虫为害所做出的防御反应比对褐飞虱的更强烈。
To explore the difference in the defense responses of rice induced by brown planthopper（BPH） Nilaparvata lugens（St？l） and white-backed planthopper（WBPH） Sogatella furcifera（Horváth）,the levels of defense-related signals, including jasmonic acid（JA）, salicylic acid（SA）, hydrogen peroxide（H_2O_2） and ethylene（ET）, and the levels of volatiles emitted from rice plants when they were infested with gravid BPH or WBPH females were investigated. The results showed that, although infestation of both gravid BPH and WBPH females increased levels of JA, SA, ET and H_2O_2 in rice plants as well as the production of rice volatiles, there were differences in the defense responses in rice induced by these two herbivores. WBPH infestation increased JA levels in rice significantly starting at 3 h and peaked at 12 h, whereas BPH infestation increased JA levels starting at 6 h and peaked at 72 h. Moreover, the levels of JA in rice plants infested by WBPH were stably higher than those in rice plants infested by BPH during 3-48 h after infestation. In addition, SA levels at 24 h, ET levels at 48 h and H_2O_2 levels at 72 h after infestation as well as the total amount of volatiles at 24 h after infestation from rice plants infested by gravid WBPH females were 1.28-, 1.45-, 4.10-, and 1.77-fold higher than those in or from rice plants infested by gravid BPH females. These results indicated that rice plants could distinguish between BPH infestation and WBPH infestation and then produce herbivore species-specific defense responses. Moreover, the defense response of rice to infestation by gravid WBPH female was quicker and stronger than that by gravid BPH females.
作者
刘晓丽
娄永根
Liu Xiaoli;Lou Yonggen(Institute of Insect Sciences,Zhejiang University,Hangzhou 310058,Zhejiang Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2018年第5期971-978,共8页
Journal of Plant Protection
基金
国家公益性行业(农业)科研专项(201403030)
国家现代农业(水稻)产业技术体系(CARS-01-40)
关键词
褐飞虱
白背飞虱
水稻
信号分子
挥发物
Nilaparvata lugens （Stal）
Sogatellafurcifera （Horvath）
rice
signaling molecule
volatile
