摘要
目的 研究肿瘤相关巨噬细胞（TAM）在乳腺癌中的浸润与血管内皮生长因子（VEGF）表达的相关性.方法 采用免疫组织化学EnVision二步法检测2014年1月至2018年1月桂林医学院附属医院45例初治乳腺癌术后组织标本中TAM标志物CD163和VEGF的表达,光学显微镜下计数TAM,半定量积分法判定VEGF的表达;分析二者与临床病理参数的关系及二者的相关性.结果 年龄≤51岁乳腺癌患者TAM浸润数量多于年龄〉51岁者[（78.1±11.9）个/高倍视野比（69.7±14.0）个/高倍视野,t=2.167,P=0.036],有淋巴结转移乳腺癌患者TAM浸润数量多于无淋巴结转移者[（79.2±11.8）个/高倍视野比（70.2±13.6）个/高倍视野,t=2.362,P=0.023].有淋巴结转移的乳腺癌患者VEGF阳性比例高于无淋巴结转移者[100.00％（20/20）比68.00％（17/25）,χ2=5.749,P=0.017].Pearson相关性分析显示,乳腺癌组织中VEGF的表达强度与TAM浸润呈正相关（r 2=0.800,P〈0.05）.结论 TAM浸润与VEGF的表达可用来预测肿瘤的恶性程度,可能是乳腺癌辅助治疗和临床预后的潜在干预靶点.
Objective To investigate the correlation between the infiltration of tumor-associated macrophages （TAM） in breast cancer and the expressions of vascular endothelial growth factors （VEGF）. Methods The expressions of CD163 （TAM marker） and VEGF in 45 postoperative tissue specimens of primary breast cancer in Affiliated Hospital of Guilin Medical University from January 2014 to January 2018 were examined by using immunohistochemistry EnVision method. Then TAM was counted under light microscope and the expression of VEGF was determined by using semi quantitative integration method. Correlation between the expression of TAM and VEGF and their relationships with clinicopathological parameters were also analyzed. Results TAM infiltration （the number of TAM under per high power field） in breast cancer patients （≤ 51 years old） was significantly more than that in breast cancer patients （〉 51 years old） [（78.1±11.9）/HP vs. （69.7±14.0）/HP, t=2.167, P=0.036]. TAM infiltration in breast cancer patients with lymph node metastasis was more than that in breast cancer patients without lymph node metastasis [（79.2 ± 11.8）/HP vs. （70.2±13.6）/HP, t= 2.362, P= 0.023]. The positive rate of VEGF in breast cancer with lymph＆nbsp;node metastasis was significantly higher than that in breast cancer without lymph node metastasis [100.00 ％（20/20） vs. 68.00％（17/25）,χ2=5.749, P=0.017]. Pearson correlation analysis showed that the expression of VEGF was positively correlated with TAM infiltration （r 2 = 0.800, P〈 0.05）. Conclusion TAM infiltration and the expression of VEGF can be used to predict the malignant degree of breast cancer, and can be used as a potential intervention target for adjuvant therapy and clinical prognosis of breast cancer.
作者
张冬冬
顾生玖
杜云龙
姚昕利
李鹤
安莉英
朱开梅
Zhang Dongdong;Gu Shengjiu;Du Yunlong;Yao Xinli;Li He;An Liying;Zhu Kaimei(Department of Analytical Medicine,Pharmacy School of Guilin Medical University,Guilin 541004,China;Department of Medicinal Chemistry,Pharmacy School of Guilin Medical University,Guilin 541004,China（Gu S J,Du YL,Li H;Department of Pathology,Affiliated Hospital of Guilin Medical University,Guilin 541001,China)
出处
《肿瘤研究与临床》
CAS
2018年第10期670-673,677,共5页
Cancer Research and Clinic
基金
广西科学研究与技术开发计划（桂科合14123001-22、桂科能1598025-46）
桂林市科学研究与技术开发计划（20150102-6、20150102-7、20150102-8）
广西植物功能物质研究与利用重点实验室开放基金（FPRU2015-5）
关键词
乳腺肿瘤
肿瘤相关巨噬细胞
血管内皮生长因子
免疫组织化学
Breast neoplasms
Tumor-associated macrophages
Vascular endothelial growth factors
Immunohistochemistry
