摘要
目的探讨宫腔粘连患者子宫内膜组织中RhoGTP酶激活蛋白29(ArhGAP29)、赖氨酰氧化酶样蛋白-2(LOXL2)及转化生长因子-β1(TGF-β1)的表达情况,并分析3个指标在宫腔粘连发病中的作用机制。方法选取2016年3月到2017年10月该院收集的76例宫腔粘连患者粘连周围的子宫内膜组织作为观察组标本,另选取该院同期收集的30例输卵管性不孕患者的正常子宫内膜组织标本作为对照组标本。采用免疫组化法检测子宫内膜组织中ArhGAP29、LOXL2及TGF-β1的表达,并分析3个指标的表达与宫腔粘连患者临床病理特征的关系以及3个指标之间的相关性。结果观察组患者子宫内膜组织中ArhGAP29的免疫组化反应评分(IRS)低于对照组,LOXL2及TGF-β1的IRS得分高于对照组,差异有统计学意义(P<0.05);宫腔粘连患者子宫内膜组织ArhGAP29表达与美国生育学会(AFS)分级、闭经有关(P<0.05);TGF-β1表达与AFS分级有关(P<0.05);LOXL2表达与刮宫次数有关(P<0.05)。ArhGAP29表达分别与LOXL2、TGF-β1表达呈负相关(P<0.05),LOXL2表达与TGF-β1表达呈正相关(P<0.05)。结论宫腔粘连患者子宫内膜组织中的ArhGAP29呈低表达,LOXL2及TGF-β1呈高表达,三者之间存在相关性,其均可能是通过影响子宫内膜纤维化进程来影响宫腔粘连的进展。
Objective To investigate the expression of RhoGTP enzyme activator protein 29 （ArhGAP29）, lysyl oxidaselike protein 2 （LOXL2） and transforming growth factor β1（TGF-β） in endometrium tissue of patient with intrauterine adhesions, and to analyze the mechanism of the three indexes in the pathogenesis of intrauterine adhesion. Methods The endometrium tissue surrounding the adhesion which was collected from 76 patients with intrauterine adhesio in the hospital from March 2016 to October 2017 was selected as speci men of the observation group ,the normal endometrium tissue which was collected from 30 patients with tubal infertility in the hospital during the same period was selected as specimens of the control group. Immunohisto chemical method was used to detect the expression of ArhGAP29,LOXL2 and TGF -β1 in the endometrium tis sue. The relationship between the expression of the three indexes and the clinicopathological features of the patients with intrauterine adhesions and the correlation between the three indexes were analyzed. Results The immuno histochemical reaction score （IRS） of ArhGAP29 in the endometrium tissue of the observation group was significantly lower than that of the control group, the IRS score of LOXL2 and TGF -β1 was significantly higher than that of the control group,and the differences were statistically significant （P〈0.05）. The expres sion of ArhGAP29 in endometrium tissue in patients with intrauterine adhesions was related to the American Fertility Society （AFS） grading and amenorrhea （P〈0.05）. The expression of TGF-β1 was related to AFSgrading （P〈0.05）. The expression of LOXL2 was related to curettage times （P〈0.05）. The expression of ArhGAP29 was negatively correlated with the expression of LOXL2 and TGF-β1 （P〈0. 05） ,and the expres sion of LOXL2 was positively correlated with the expression of TGF -β1（P〈0.05）. Conclusion ArhGAP29 in endometrium tissue of patients with intrauterine adhesions is low expressed, and LOXL2 and TGF -β1 are high ly expressed. There are correlations between the three indexes,which may affect the progress of endometrium adhesion by affecting the process of endometrial fibrosis.
作者
徐力
彭娟
张荣华
XU Li;PENG Juan;ZHANG Ronghua(Department of Gynaecology and Obstetrics,Chengdu Xindu District People＇s Hospital,Chengdu,Sichuan 610500,China)
出处
《国际检验医学杂志》
CAS
2018年第22期2762-2766,共5页
International Journal of Laboratory Medicine
基金
四川省医学科研青年创新课题资助项目(Q15067)
