摘要
目的：研究绞股蓝总黄酮抗心肌缺血保护作用及其抗氧化应激反应研究。方法：40只大鼠随机分为假手术组、模型组、绞股蓝总黄酮组、生理盐水组,每组10只,假手术组只穿线不结扎,其他组大鼠进行心肌缺血再灌注损伤造模。造模24 h后,测定各组大鼠心肌梗死面积;测定血清心肌酶（LDH、CK、GOT）活力、氧化应激指标（MDA、SOD、GSH-Px和CAT）表达水平;测定心肌组织中氧化应激指标（MDA、SOD、GSH-Px和CAT）表达水平。结果：与假手术组相比,模型组心肌梗死面积显著增大（P 〈0. 05）,血清LDH、CK、GOT活力显著升高（P 〈0. 05）,血清和心肌组织中MDA水平显著升高（P 〈0. 01）,SOD、CAT和GSH-Px活力显著下降（P 〈0. 01）。与模型组和生理盐水组相比,绞股蓝总黄酮组心肌梗死面积明显减小（P 〈0. 05）,血清LDH、CK、GOT活力显著升高（P 〈0. 05）,血清和心肌组织中MDA水平显著升高（P 〈0. 01）,SOD、CAT和GSH-Px活力显著下降（P 〈0. 01）。结论：绞股蓝总黄酮可以显著改善缺血再灌注大鼠氧化应激反应,减少心肌梗死面积,起到保护心肌作用。
Objective：To study the protective effect of total flavones from Gynura divaricata against myocardial ischemia and its anti-oxidative stress response. Methods： Forty rats were randomly divided into sham operation group,model group,total gypenosides group and normal saline group,with 10 rats in each group. Sham-operated rats were not ligated by threading. The other rats were subjected to myocardial ischemia-reperfusion injury model. After 24 h of modeling,myocardial infarct size was determined in each group. The activities of serum myocardial enzymes（ LDH,CK,GOT） and the expression of oxidative stress indicators（ MDA,SOD,GSH-Px and CAT） expression levels were compared. Results：Compared with the sham operation group,the area ofmyocardial infarction in model group was significantly increased（ P〈0. 05）;the activities of serum LDH,CK and GOT were significantly increased（ P〈0. 05） and the levels of MDA in serum and myocardial tissue were significantly increased（ P〈0. 01）. The activities of SOD,CAT and GSH-Px were significantly decreased（ P〈0. 01）. Compared with the model group and the saline group,the myocardial infarct size in the total flavonoids of Gynostemma pentaphyllum decreased significantly（ P〈0. 05）; the activities of serum LDH,CK and GOT increased significantly（ P〈0. 05） and MDA levels in serum and myocardium significantly increased（ P〈0. 01）.The activities of SOD,CAT and GSH-Px decreased significantly（ P〈0. 01）. Conclusion： The total flavonoids of Gynostemma can significantly improve the oxidative stress response of rats with ischemia-reperfusion,reduce the area of myocardial infarction and plays a role in protecting myocardium.
作者
孙立峰
郭华
孟剑锋
张保英
王建华
SUN Lifeng;GUO Hua;MENG Jianfeng;ZHANG Baoying;WANG Jianhua(Cardiac Surgery Department,Xingtai People＇ s Hospital,Xingtai 054001,Hebei,China;Neonatology Department,Xingtai Third Hospital,Xingtai 054000,Hebei,China)
出处
《中华中医药学刊》
CAS
北大核心
2018年第10期2513-2515,共3页
Chinese Archives of Traditional Chinese Medicine
基金
河北省自然科学基金项目(C2011206048)
关键词
绞股蓝总黄酮
氧化应激
心肌缺血
心肌保护
Gynostemma flavonoids
oxidative stress
myocardial ischemia
myocardial protection
