摘要
采用DPPH自由基(DPPH·)清除、ABTS自由基(ABTS+·)清除、Cu2+还原及PTIO自由基(PTIO·)清除(p H 4. 5和p H 7. 4)法,评价异荭草苷的抗氧化活性,探讨其可能的机制.以上5个抗氧化分析法中,异荭草苷均表现出良好的量效关系,其IC50依次为(6. 5±0. 2,2. 0±0. 1,11. 0±0. 0,637. 3±46. 6,(508. 4±11. 4)μmol·L-1.这些IC50值均低于标准抗氧化剂Trolox的IC50值[IC50值分别为11. 6±0. 8,3. 8±0. 1,38. 0±0. 6,668. 0±21. 5,(692. 5±32. 9)μmol·L-1].这表明异荭草苷具有良好的抗氧化活性,这种活性可能是通过氢转移(HAT,hydrogen-atom-transfer)或电子转移(ET,electron-transfer)完成的.异荭草苷HAT的能力可能强于ET的能力.
The antioxidant activity of isoorientin was evaluated by several antioxidant assays,including DPPH ·-scavenging assay, ABTS＋·-scavenging assay,cupric ions reducing assay,and PTIO·-scavenging assay at pH 4. 5 and p H 7. 4. In the above five experiments,isoorientin exhibited a good dose-response manner. The IC50 values of isoorientin were 6. 5 ± 0. 2,2. 0 ± 0. 1,11. 0 ± 0. 0,637. 3 ± 46. 6,and（508. 4 ± 11. 4） μmol · L^-1,respectively. These IC50 values were lower than those of Trolox [IC50= 11. 6 ± 0. 8,3. 8 ± 0. 1,38. 0 ± 0. 6,668. 0 ± 21. 5,（692. 5 ± 32. 9）μmol·L^-1]. The comparison suggested that isoorientin had a good antioxidant activity in vitro. It＇ s antioxidant action is proposed to be fulfilled through HAT（ hydrogen-atomtransfer） or ET（ electron-transfer）. However,its HAT possibility may be higher than ET possibility.
作者
江倩
黄燕芳
李熙灿
JIANG Qian;HUANG Yan-fang;LI Xi-can(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第5期50-55,共6页
Journal of Fujian Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(81573558)
广东省自然科学基金资助项目(2017A050506043)
