摘要
建立一种HPLC同步测定洋金花中东莨菪碱和莨菪碱含量的方法。采用SGE protecol C18(5μm,4.6 mm×250 mm)色谱柱,流动相为乙腈-30 mmol/L醋酸铵溶液(含0.02%三乙胺、0.3%四氢呋喃,用冰醋酸调pH至6.0)(10∶90,V/V),检测波长为216 nm,流速1.0m L/min,柱温30℃,进样量20μL。东莨菪碱和莨菪碱的线性范围分别为56.875～910μg和75.625～1210μg,且分离度高,重复性、精密度、稳定性及回收率均符合要求。本方法简单、准确,可用于洋金花中东莨菪碱和莨菪碱的含量测定及质量控制。
This research was to establish a method for determination of scopolamine and hyoscyamine in Datura metel byHPLC at the same time.SGE protecol C18（5μm, 4.6 mm×250 mm）chromatographic column was used, with mobile phaseconsist of acetonitrile-30 mmol/L ammonium acetate（containing 0.02% triethylamine, 0.3% tetrahydrofuran, and the pH wasadjusted to 6.0 with glacial acetic acid）（10：90, V/V） , the detective wavelength was 216 nm, the flow rate was 1.0 mL/min andthe column temperature was 30℃. The linear range of scopolamine and hyoscyamine were 56.875～910 μg and 75.625～1210μg, respectively, and the separation of components was better, the repeatability, precision, stability and recovery of themethod were favorable. The established method is sample, accurate and suitable for the determination and quality control ofDatura metel.
作者
李红红
陈朋朋
付传香
吴泽宇
惠爱玲
许坤
张文成
LI Hong-hong;CHEN Peng-peng;FU Chuan-xiang;WU Ze-yu;HUI Ai-ling;XU Kun;ZHANG Wen-cheng(Engineering Research Center of Bio-process from Ministry of Education,College of Food Science and Engineering,HefeiUniversity of Technology,Hefei 230009,China;Anhui Dexinjia Biological Medicine Co.Ltd.,Fuyang 236000,China)
出处
《安徽化工》
CAS
2018年第5期92-94,共3页
Anhui Chemical Industry
基金
安徽省科技重大专项项目(16030801111)资助
关键词
洋金花
东莨菪碱
莨菪碱
HPLC
含量测定
Datura metel
scopolamine
hyoscyamine
HPLC
content determination
