摘要
为了解猪内源性反转录病毒(Porcine endogenous retrovirus, PERV)在海南地方猪种五指山猪和海南猪基因组中的存在状况。试验利用猪内源性反转录病毒特异性引物对这两种猪的基因组进行PCR检测。结果显示:PERV普遍存在于海南地方猪基因组中,其中在五指山猪中PERV-A、PERV-B、PERV-C存在率分别是100%、100%、20.69%;在海南猪中PERV-A、PERV-B、PERV-C存在率分别是100%、100%、25.49%。该研究为海南地方猪的开发、利用及其病毒安全性评价奠定了基础。
In order to investigate the status of the porcine endogenous retrovirus in the genome of Hainan native pig breeds, Wuzhishan pig and Hainan pig, specific primers were set to detect the genomes of these two pigs. The results showed that PERV was common among genomes of Hainan native pig breeds. In Wuzhishan pig, the presence rates of PERV-A, PERV-B and PERV-C were 100%, 100% and 20.69%, respectively. In Hainan pig, the presence rates of PERV-A, PERV-B and PERV-C were 100%, 100% and 25.49%, respectively. This research may be helpful to utilize the Hainan native pigs and to assess the biosafety of PERV.
作者
晁哲
吴望军
刘海隆
孙瑞萍
王峰
郑心力
CHAO Zhe;WU Wangjun;LIU Hailong;SUN Ruiping;WANG Feng;ZHENG Xinli(Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China;Hainan Provincial Engineering Research Center for Livestock and Poultry Cultivation,Haikou 571100,China;College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
出处
《养猪》
2018年第5期97-99,共3页
Swine Production
基金
海南自然科学基金创新研究团队项目(2017CXTD013)
