摘要
目的运用细胞代谢呼吸动态分析仪研究口腔鳞癌相关成纤维细胞(oral cancer associated fibroblasts,CAFs)能量代谢表型,为靶向CAFs代谢治疗口腔鳞癌提供研究依据。方法原代培养CAFs和癌旁正常成纤维细胞(normal fibroblasts,NFs),细胞代谢呼吸动态分析仪对CAFs和NFs进行线粒体和糖酵解压力测试,通过比较CAFs和NFs的OCR、ECAR值分析二者氧化磷酸化和糖酵解代谢水平的差异,得出CAFs能量代谢表型。结果 CAFs和NFs氧化磷酸化基础水平基本一致,CAFs氧化磷酸化最大值和储备值明显提高(P<0.01);CAFs糖酵解基础值、最大值和储备值均明显高于NFs(P<0.01)。结论 CAFs氧化磷酸化和糖酵解代谢能力较NFs显著增强,氧化磷酸化水平的增强主要体现在储备能力上,抑制CAFs氧化磷酸化储备能力可能成为新的抑癌靶点。
Objective To investigate the metabolic phenotype of oral cancer associated fibroblasts. Methods The oral cancer associated fibroblasts（ CAFs） and normal fibroblasts（ NFs） were cultured in vitro. XF cell mito stress test and XF glycolysis stress test were performed on CAFs and NFs by XFe96 extracellular flux analyzer. Differences of oxidative phosphorylation and glycolysis metabolism between CAFs and NFs were analyzed by comparing the values of OCR and ECAR,and the metabolic phenotype of CAFs was obtained. Results OCR values representing maximal respiration and spare respiratory capacity of CAFs were greatly enhanced compared with NFs（ P〈0. 01）,while OCR values representing basal respiration level were almost the same between CAFsand NFs. ECAR values representing glycolysis,glycolytic capacity and glycolytic reserve of CAFs were significantly higher than those of NFs（ P〈0. 01）. Conclusion The capacities of oxidative phosphorylation and glycolysis of CAFs were significantly increased than those of NFs. The enhancement of oxidative phosphorylation level was mainly due to the higher spare respiratory capacity,so inhibiting the spare respiratory capacity of CAFs may become a new target for oral cancer suppression.
作者
任倩
冯芝恩
杨彬
关晓兵
REN Qian;FENG Zhi-en;Yang Bin;GUAN Xiao-bing(Department of Oral Medicine,Capital Medical University School of Stomatology,Beijing 100050,China)
出处
《北京口腔医学》
CAS
2018年第4期196-200,共5页
Beijing Journal of Stomatology
基金
北京市自然科学基金青年项目(7154203)
北京口腔医院学科建设基金基础专项(15-09-01)
关键词
口腔鳞癌相关成纤维细胞
氧化磷酸化
糖酵解
Oral cancer associated fibroblasts
Oxidative phosphorylation
Glycolysis
