摘要
目的:研究沉默信息调节因子2(SIRT2)对骨关节炎软骨组织中炎症反应、骨质破坏的影响。方法:选择因膝关节骨性关节炎在喀什地区第一人民医院接受膝关节置换术的200患者作为研究的OA组,另取同期因外伤在喀什地区第一人民医院接受膝关节置换术、半月板手术的80例患者作为对照组。术后采集关节软骨组织并测定SIRT2、骨破坏相关细胞凋亡分子的表达量以及炎症反应分子、骨破坏相关胶原代谢分子的含量。结果:OA组患者关节软骨组织中SIRT2、Bcl-2的mRNA表达量以及SOX9、Col-Ⅱ的含量显著低于对照组,TNF-α、bFGF、NO、IP-10、CCL2、PAR-2、β-catenin、OPN、MMP13的含量以及Fas、GRP78、ATF6、Caspase-3的mRNA表达量均显著高于对照组;OA组患者关节软骨组织中SIRT2的mRNA表达量与Bcl-2的mRNA表达量以及SOX9、Col-Ⅱ的含量呈正相关,与TNF-α、bFGF、NO、IP-10、CCL2、PAR-2、β-catenin、OPN、MMP13的含量以及Fas、GRP78、ATF6、Caspase-3的mRNA表达量呈负相关。结论:骨关节炎软骨组织中低表达的SIRT2能够加剧炎症反应及骨质破坏。
Objective：To study the effects of silent information regulator of transcription 2（SIRT2）on inflammatory response and bone destruction in cartilage tissue of osteoarthritis.Methods：200 patients who underwent knee replacement due to knee osteoarthritis in Kashgar Prefecture First People＇s Hospital between September 2014 and September 2017 were selected as the OA group of the research,and 80 patients who underwent knee replacement or meniscus operation due to trauma in Kashgar Prefecture First People＇s Hospital during the same period were selected as the control group.Articular cartilage tissue was collected after surgery to measure the expression of SIRT2 and bone destruction-related apoptosis molecules as well as the levels of inflammatory response molecules and bone destruction-related collagen metabolism molecules.Results：SIRT2 and Bcl-2 mRNA expression as well as SOX9 and Col-Ⅱ levels in articular cartilage tissue of OA group were significantly lower than those of control group whereas TNF-α,bFGF,NO,IP-10,CCL2,PAR-2,β-catenin,OPN and MMP13 levels as well as Fas,GRP78,ATF6 and Caspase-3 mRNA expression were significantly higher than those of control group;SIRT2 mRNA expression in articular cartilage tissue of OA group was positively correlated with Bcl-2 mRNA expression as well as SOX9 and Col-Ⅱ levels,and negatively correlated with TNF-α,bFGF,NO,IP-10,CCL2,PAR-2,β-catenin,OPN and MMP13 levels as well as Fas,GRP78,ATF6 and Caspase-3 mRNA expression.Conclusion：The lowly expressed SIRT2 in cartilage of osteoarthritis can aggravate inflammatory response and bone destruction.
作者
艾克白尔.吐逊
帕孜拉.艾拉
麦麦提热夏提.米吉提
阿吉木.克热木
TUERXUN Aikebaier;AILA Pazila;MIJITI Maimaitirexiati;KEREMU Ajimu(Department of Traumatic Orthopedics,Kashgar Prefecture First People＇s Hospital in Xinjiang,Kashgar,Xinjiang,844000,China)
出处
《海南医学院学报》
CAS
2018年第14期1327-1330,共4页
Journal of Hainan Medical University
基金
喀什地区科学研究与技术开发计划项目(KS2017050)~~
关键词
骨关节炎
沉默信息调节因子2
炎症反应
骨质破坏
osteoarthritis
silent information regulator of transcription 2
inflammatory response
bone destruction
