摘要
目的通过建模研究α1-抗胰蛋白酶(AAT)治疗糖尿病的动物药效学。方法将大鼠造成Ⅱ型糖尿病模型,选择血糖值在15~30 mmol/L者用于实验组,AAT分为高(11.5 mg/kg)、低(5.75 mg/kg)两个剂量组,盐酸二甲双胍缓释片做阳性对照。测定血清血糖(GLU)、肌酐(Cre)、尿素氮(BUN)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),并进行病理组织学检测。结果与模型组比较,二甲双胍组、AAT低剂量组、AAT高剂量组中GLU均明显降低,差异有统计学意义(P<0.05);TP在二甲双胍组、AAT低剂量组有升高趋势,在AAT高剂量组有降低趋势;三个受试药组TC明显降低;TG在二甲双胍组有升高趋势,在AAT低、高剂量组中均明显降低(P<0.05)。结论 AAT对Ⅱ型糖尿病大鼠模型有明显的降糖、降脂作用,AAT低剂量对TP有升高的趋势,可改善实验动物肾脏和胰腺内胰岛形态的作用。
Objective The pharmacodynamics of α1-antitrypsin（AAT）in the treatment of diabetes mellitus was studied by modeling.Methods Model rats with type 2 diabetes with blood glucose level of 15~30 mmol/L were selected as the experimental group.High dose group was treated with AAT 11.5 mg/kg and the low dose group was was treated with AAT5.75 mg/kg.The positive control group was treated with metformin hydrochloride sustained-release tablet.The serum levels of glucose（GLU）,creatinine（Cre）,urea nitrogen（BUN）,total protein（TP）,albumin（ALB）,globulin（GLB）,triglyceride（TG）,total cholesterol（TC）,high density lipoprotein cholesterol（HDL-C）and low density lipoprotein cholesterol（LDLC）were assyed.Histopathological examination was also performed.Results Compared with the model group,the GLU was significantly lower in the metformin group,AAT low-dose group,and AAT high-dose group,differences are statistically significant（P〈0.05）.TP increased in the metformin group and AAT low-dose group,and decreased in the AAT high-dose group.TC decreased significantly in the three trial groups.TG increased in the metformin group,and significantly decreased in the AAT low-dose group and AAT high-dose group.Conclusion The results showed that AAT had obvious hypoglycemic and lipid-lowering effects on type 2 diabetic rats,and low dose of AAT had a tendency to increase the total protein,which could improve the kidney and pancreaticislet morphology in the experimental animals.
作者
陈献雄
张英
蒋伟
张利
王妍
CHEN Xian-xiong;ZHANG Ying;JIANG Wei;ZHANG Li;WANG Yan(Shenzhen University Health Science Center,Shenzhen,Guangdong 518055;College of Applied Chemistry and Biotechnology,Shenzhen Polytechnic,Shenzhen,Guangdong 518055,China)
出处
《热带医学杂志》
CAS
2018年第7期869-872,918,F0002,共6页
Journal of Tropical Medicine
基金
深圳市科技创新委(JCYJ20160527172247868)
深圳市大规模细胞培养技术与细胞资源库公共技术服务平台(GGJS20130331152344401)
横向课题
