摘要
目的观察黄芪百合颗粒对亚硝酸钠(NaNO2)中毒小鼠存活时间、肺、心脑组织病理形态学、过氧化氢酶(CAT)和三磷酸腺苷(ATP)的影响。方法将60只SPF级KM种雄性小鼠常规饲养3d后随机分组,空白组和模型组均灌胃生理盐水,阳性对照组给予红景天枸杞胶囊液,黄芪百合颗粒低、中、高剂量组分别按照1.75、3.5、7mg/(kg·d)剂量灌胃,1次/d,连续30d。末次给药1h后,除空白对照组外,其余各组按200mg/kg腹腔注射亚硝酸钠,注射量为0.2mL。记录小鼠存活时间;HE染色观察肺、心、脑组织的病理形态学改变;采用比色分析法测定肺、心、脑组织中过氧化氢酶(CAT)活性和三磷酸腺苷(ATP)含量。结果与空白组比较,模型组小鼠存活时间明显降低[(9.64±1.60)min,P<0.05];肺泡组织出现扩张、融合,肺间质内红细胞渗出,肺泡壁受压变窄;心肌间质血管扩张充血严重,心外膜下局部细胞水肿变性;脑组织出现脑神经细胞坏死,核固缩,且出现核周空泡现象,组织疏松,血管及神经细胞周围间隙增宽;肺、心、脑组织CAT活性降低(P<0.05),ATP含量降低(P<0.05);与模型组比较,黄芪百合颗粒低[(12.78±2.20)min]、中剂量组[(13.22±2.10)min]小鼠存活时间明显延长(P<0.05),黄芪百合颗粒低、中、高剂量组小鼠肺泡组织扩张、肺间质红细胞渗出均不同程度减轻,心间质血管扩张充血不同程度减轻,脑组织神经细胞、核固缩现象不同程度减轻;肺、心、脑组织CAT活性增强(P<0.05)、ATP含量升高(P<0.05)。结论黄芪百合颗粒对小鼠NaNO2中毒性缺氧损伤具有保护作用。
Objective To study the effect of Astragali Lilium Granules on the survival time,CAT,ATP,and pathological changes in mice exposed to sodium nitrite（NaNO2）poisoning.Methods We randomly divided 60 male mice of SPF grade KM into blank group,positive control group,model group and Astragali Lilium Granules low-,medium-and high-dose groups.After 3 days of normal feeding,blank group and model group received intragastric gavage of normal saline,and positive control group was given Rhodiola rosea capsule solution.Astragali Lilium Granules groups received intragastric gavage according to the concentration（1.75,3.5 and7 mg/kg per day）for 30 days. One hour after the last administration,all groups except the blank control group received intraperitoneal injection of NaNO2（200 mg/kg,injection amount of 0.1 mL/g）.We recorded the mice＇s survival time,observed the pathological changes of brain,lung and heart tissues by HE staining under the microscope.The activity of CAT and the level of ATP in brain tissue were determined by colorimetric analysis.Results Compared with the blank group,the survival time in model group [（9.64±1.60）min,P〈0.05]was significantly lower;in model group,the lung tissue showed extensive pulmonary edema,red cell exudation,emphysema,and local atrophy of the lung;the interstitium of the myocardium was dilated and congested,and the local cells in the epicardium became edematous and denatured;brain cells showed necrosis,cytoplasm condensation,pyknosis,and perinuclear vacuoles.The tissues were loose and the spaces between the vessels and nerve cells widened;the activity of CAT decreased and the content of ATP decreased in lung,heart and brain tissues（P〈0.05）.Compared with the model group,the survival time was significantly prolonged in Astragali Lilium Granules low-[（12.78±2.20）min]and medium-dose[（13.22±2.10）min]groups（P〈0.05）.Compared with model group,the three dose groups had lessened lung tissue edema and emphysema,cardiac interstitial vascular dilatation and congestion,necrosis and nuclear pyknosis of brain nerve cells;the activity of CAT increased and the content of ATP increased in lung,heart and brain tissues（both P〈0.05）.Conclusion Astragali Lilium Granules can improve the toxicity of NaNO2 in mice and thus effectively protect against NaNO2-induced anoxic injury.
作者
张丽昕
苏韫
颜春鲁
刘永琦
张苡铭
陈刚
冯彩琴
成新平
ZHANG Li-xin;SU Yun;YAN Chun-lu;LIU Yong-qi;ZHANG Yi-ming;CHEN Gang;FENG Cai-qin;CHENG Xin-ping(Gansu University of Chinese Medicine Gansu Provincial Key Laboratory of Major Disease Prevention and Control Research of Molecular Medicine and Traditional Chinese Medicine,Lanzhou 730000;Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Levels,Lanzhou 730000,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2018年第4期602-608,共7页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陇原青年创新人才扶持计划(No.2014-93-72)
甘肃省高校重大疾病分子医学与中医药防治重点实验室开放基金(No.ZYX15-16-3)
2012甘肃省科技支撑计划项目(No.120FKCA169)
