摘要
目的:探讨不同剂量玛咖对力竭运动致大鼠运动性低血糖的保护作用。方法:采用递增负荷力竭游泳训练的方法建立运动性低血糖动物模型。55只42 d龄雄性Wistar大鼠随机分为5组:(1)静止对照组(C组),(2)运动对照组(M组),(3)^(5)运动+低、中、高剂量玛咖组(LM I、LM II,LM III组),每组10只(剔除不符合实验要求的大鼠5只)。每天灌胃(ig)1次,LM各组灌胃玛咖的剂量为0.2,0.4,1.2 g/kg体重,灌胃体积为5 ml/kg体重,C、M组灌胃等体积生理盐水。运动大鼠采用递增负荷力竭游泳训练42 d,42 d力竭游泳训练后,测定体重、力竭游泳时间,取血、肝脏及深层股四头肌检测相关生化指标。结果:与C组比较,M组体重、血糖水平、肌糖原与肝糖原含量、肝细胞磷酸烯醇式丙酮酸羧激酶(phosphoenol pyruvate carboxy kinase,PEPCK)表达、肝细胞阳性染色累计吸光度均值等均明显降低(P<0.05或P<0.01);力竭游泳时间无明显差异;血乳酸含量明显升高(P<0.01)。与M组比较,LM各组体重、血糖水平、肌糖原与肝糖原含量、肝细胞PEPCK表达、肝细胞阳性染色累计吸光度均值等均明显升高(P<0.05或P<0.01),力竭游泳时间明显延长(P<0.01),血乳酸含量明显降低(P<0.01)。与LM I组比较,LMⅢ组体重无明显差异,LM III组血糖水平、肌糖原与肝糖原含量、肝细胞PEPCK表达、肝细胞阳性染色累计吸光度均值等均明显升高(P<0.05),力竭游泳时间明显延长,血乳酸含量明显降低(P<0.05)。结论:高剂量玛咖可有效抑制和延缓长时间、大负荷运动导致的运动性低血糖和运动性疲劳的发生与发展,其机制可能与优化肌糖原和肝糖原的储备量及上调糖异生限速酶PEPCK的表达,提高PEPCK活性,促进糖异生有关。
Objective： To investigate the protective effects of different dosage of L. meyennii on hypoglycemia of rats induced by exhaustive exercise. Methods： The exercise-induced hypoglycemia animal model was established using the increasing load to exhaustion swimming training. Fifty-five male Wistar rats aged six-week were randomly divided into five groups：（1）control group（ C group）,（2）exercise control group（ M group）,（3） -（5）exercise ＋ low dose or medium dose or high dose L. meyennii group（ LM I,LM II,LM III group）,10 rats in each group（ 5 rats were excluded）. L. meyennii was given to LM groups once a day,the dosage was 0. 2,0. 4,1. 2 g/kg body weight respectively,the volume was 5 ml/kg body weight,C and M group were given the same volume of normal saline intragastrically. The exercise rats were drilled in an exhaustive swimming training for 42 d with an increasing load. After 42 d exhaustive training,the body weight,exhaustive swimming time were determined,and related biochemical indicators were detected from samples of plasma,liver and deep strands of quadriceps. Results： The body weight,the level of blood glucose,muscle glycogen,hepatic glycogen,expression of PEPCK in hepatocytes and hepatocyte positive staining accumulated absorbance of M group were lower than those of C group（ P 〈0. 05 or P 〈0. 01）,there was no obvious difference between C and M groups in the time of exhaustive swimming. Compared with the C group,the content of blood lactate of M group was increased significantly（ P 0. 01）. Compared with M group,body weight,the level of blood glucose,muscle glycogen,hepatic glycogen,expression of PEPCK in hepatocytes and hepatocyte positive staining accumulated absorbance of LM groups were obviously increased（ P 〈0. 05 or P 〈0. 01）,the time of exhaustive swimming was prolonged（ P 〈0. 01）,and the content of blood lactate was decreased significantly（ P〈 0. 01）. There was no difference between LM Ⅰ and LM III group in body weight. Compared with LM I group,the level of blood glucose,muscle glycogen,hepatic glycogen,expression of PEPCK in hepatocytes and hepatocyte positive staining accumulated absorbance of LM Ⅲ group was obviously increased（ P〈 0. 05）,the time of exhaustive swimming of LM Ⅲ group was prolonged,and the content of blood lactate of LM Ⅲ group decreased significantly（ P 〈0. 05）. Conclusion： High dosage of L. meyennii may effectively suppress and delay the occurrence and development of exercise-induced hypoglycemia and fatigue induced by the long-time and intensive training,and the mechanism may be related to optimize of muscle glycogen and hepatic glycogen reserve in one hand. It is also related to up-regulates the limited enzyme of PEPCK expression and activity in the other hand. Finally it promotes the impact of gluconeogenesis.
作者
王彦英
曹建民
周海涛
张静
牛衍龙
王灿
李林蔚
李思琪
WANG Yan-ying;CAO Jian-min;ZHOU Hai-tao;ZHANG Jing;NIU Yan-long;WANG Can;LI Lin-wei;LI Si-qi(Beijing Wuzi University,Beijing 101149;Beijing Sport University,Beijing 100084;Biochemical Engineering College of Beijing Union University,Beijing 100023,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2018年第3期229-233,共5页
Chinese Journal of Applied Physiology
基金
北京市朝阳区协同创新项目(CYXC1508)
北京联合大学"启明星"大学生科技创新项目(201611417022)
关键词
玛咖
力竭运动
运动性低血糖
限速酶
磷酸烯醇式丙酮酸羧激酶
大鼠
Lepidium meyennii
exhaustive exercise
exercise-induced hypoglycemia
rate-limiting enzyme
phosphoenolpyruvate carboxykinase
rat
