摘要
目的探讨抑制口腔鳞状细胞癌(OSCC)中半乳糖凝集素-3(Galectin-3)基因的表达对癌细胞增殖、侵袭、凋亡的影响及机制。方法逆转录聚合酶链反应(RT-PCR)和蛋白质印迹(Western blotting)分别检测OSCC中Galectin-3 m RNA和蛋白表达;人OSCC Tca8113分为空白组、阴性对照组和Galectin-3转染组,转染48 h后,Western blotting检测各组细胞中Galectin-3、基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)、活化的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved Caspase-3)、β-连环蛋白(β-catenin)、细胞周期素D1(Cyclin D1)蛋白表达;CCK8检测细胞增殖;Transwell小室检测细胞侵袭能力;流式细胞术检测细胞凋亡。结果 OSCC中Galectin-3m RNA及蛋白表达均显著高于癌旁组织(P<0.01);RNA干扰Galectin-3表达后Tca8113细胞中Galectin-3蛋白表达明显降低;Galectin-3转染组细胞存活率、细胞侵袭能力及MMP-2、MMP-9、β-catenin、Cyclin D1蛋白表达均显著低于空白组,细胞凋亡率及Cleaved Caspase-3蛋白表达均显著高于空白组(P<0.01)。结论抑制OSCC中Galectin-3基因表达可显著降低癌细胞的增殖及侵袭能力,诱导细胞凋亡,其机制与下调Wnt/β-catenin信号通路有关。
Objective The effect and mechanism of Galectin-3 gene expression on proliferation, invasion, and apoptosis of oral squamous cell carcinoma（OSCC） were investigated. Methods Reverse transcription-polymerase chain reaction（RTPCR） and Western blotting were used to detect the m RNA and protein of Galectin-3 gene in OSCC. OSCC Tca8113 was divided into control, negative control, and Galectin-3 transfection groups. Western blotting was used to detect the expression of Galectin-3, matrix metalloproteinase（MMP）-2, MMP-9, Cleaved Caspase-3, β-catenin, and Cyclin D1 protein after transfection for 48 h in each group. Cell proliferation was detected by CCK8. Cell invasion ability was detected by using a Transwell chamber. Cell apoptosis was detected by flow cytometry. Results The m RNA and protein expression levels of Galectin-3 gene in OSCC were significantly higher than those in adjacent tissues（P0.01）. Galectin-3 protein expression in Tca8113 cells significantly decreased after RNA interference. Cell survival rate and invasion as well as MMP-2, MMP-9, β-catenin, and Cyclin D1 protein expression were significantly lower than the blank group. Apoptosis rate and Cleaved Caspase-3 protein expression were significantly higher than the control group（P0.01）. Conclusion Inhibition of Galectin-3 gene expression in OSCC can significantly reduce the proliferation and invasion of cancer cells and induce apoptosis. The mechanism is related to downregulation of the Wnt/β-catenin signaling pathway.
作者
方政
邱峰
赵军方
孙强
乔彬
李光辉
李新明
Fang Zheng;Qiu Feng;Zhao Junfang;Sun Qiang;Qiao Bin;Li Guanghui;Li Xinming(Dept.of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Chin)
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2018年第4期404-409,共6页
West China Journal of Stomatology
基金
国家自然科学基金(8140100298)~~
