摘要
以15份稳定遗传的转CarNAC1基因棉花及受体材料K62进行田间和室内试验,通过加权隶属函数法、主成分分析法及聚类分析等方法比较棉花光合指标、农艺性状、产量性状、纤维品质和生理指标的变化情况。结果表明:干旱胁迫后,转基因棉花品系气孔导度下降趋势及净光合速率显著高于受体材料K62;转基因棉花品系平均单铃重高于受体材料0.363~0.657 g;除丙二醛含量为转基因棉花品系低于受体材料K62外,转基因棉花品系的脯氨酸、可溶性糖含量及过氧化氢酶、过氧化物酶、超氧化物歧化酶活力均高于受体材料。基于农艺性状和产量性状指标主成分分析结果,将16个品系的9个指标综合为2个相互独立的综合指标,结合D值及聚类分析结果筛选出干旱胁迫后仍能保持相对较高产量的品系:ZK62-10、ZK62-6、ZK62-1。基于纤维品质指标主成分分析结果,将16个品系的7个指标综合为3个相互独立的综合指标,结合D值及聚类分析结果筛选出干旱胁迫后仍能保持相对较高纤维品质水平的品系:ZK62-6、ZK62-12、ZK62-15。可以得出以下结论:干旱胁迫下,转CarNAC1基因棉花品系的产量及品质均优于受体材料K62;转CarNAC1基因棉花品系可能通过降低气孔导度、增强渗透条件等方法提高棉花抗旱能力;筛选出综合抗旱能力最优的棉花品系ZK62-6。
In this paper,15 stable gebetically transgenic cotton plants with CarNAC1 gene and the receptor materials K62 were used for filed and laboratory tests,cotton photosynthesis index,agronomic traits,yield traits and fiber quality and physiological indicators were compared by weighted membership function method,principal component analysis and cluster analysis. The results showed that after drought stress,the stomatal conductance decreased and net photosynthetic rate of the transgenic cotton lines was significantly higher than that of the receptor material K62. the average boll weight of the transgenic cotton lines was higher than that of the receptor material 0.363 ~ 0. 657 g; In addition the content of MDA was lower than the receptor material of K62 transgenic cotton lines,proline,soluble sugar content and catalase,peroxidase and superoxide dismutase activity in transgenic cotton lines were higher than that of the receptor material. Based on the results of principal components analysis of agronomic traits and yield traits indicators,the 9 indexes of 16 lines were integrated into 2 independent comprehensive indexes,which combined with D value and cluster analysis results can still maintain relatively high yield after drought stress. The lines： ZK62-10,ZK62-6,ZK62-1. Baesed on the results of principal components analysis of fiber quality index,the 7 indicators of 16 lines were integrated into 3 independent comprehensive indexes,combined with D value and cluster analysis results were used ro screen out the relatively high fiber quality levels after drought stress. the lines： ZK62-6,ZK62-12,ZK62-15. After drought stress,the yield and quality of transgenic cotton lines carrying CarNAC1 gene were better than that of receptor K62. CarNAC1 transgenic cotton lines may improve the drought resistance of cotton by reducing stomatal conductance and enhancing the osmotic conditions. The best cotton line with comprehensive drought resistance ability was screened out： ZK62-6.
作者
王淑君
曲延英
倪志勇
王莉萍
高文伟
陈全家
WANG Shu-jun, QU Yan-ying, NI Zhi-yong, WANG Li-ping, GAO Wen-wei, CHEN Quan-jia(College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 83005)
出处
《干旱地区农业研究》
CSCD
北大核心
2018年第4期272-281,共10页
Agricultural Research in the Arid Areas
基金
国家转基因生物新品种培育重大专项(2016ZX08005-004-009)
