摘要
目的回顾性研究北京大学第一医院2007-2017年分离的诺卡菌的感染途径、菌种分布和药敏情况。方法纳入北京大学第一医院从2007年1月至2017年7月住院患者标本分离得到的10株诺卡菌,使用高分辨飞行时间质谱MALDI-TOF进行菌种鉴定,进行16sRNA和rpoB测序。用etest试纸法进行药敏试验。结果 10株诺卡菌,分别是4株盖尔森基兴诺卡,3株脓肿诺卡,2株巴西诺卡和1株皮疽诺卡。其中,4株分离自痰液,2株分离自肺泡灌洗液,肺脓肿脓液、皮肤疖肿、关节腔液和腹腔引流液各有1株。10株诺卡菌的药物敏感度最高的是利奈唑胺、复方磺胺甲噁唑和阿米卡星,均在100%;庆大霉素和头孢曲松可以达到90%,亚胺培南只有40%;最低的是环丙沙星(0%)和克林霉素(10%)。结论分离的诺卡菌以盖尔森基兴诺卡和脓肿诺卡为主,肺部感染是主要感染方式;最敏感的药物为复方磺胺甲噁唑、阿米卡星和利奈唑胺。
Objective To retrospectively study the infection route,bacterial strain distribution and drug susceptibility of isolated nocardia in the First Hospital of Peking University.Methods Ten strains of norcadia isolated from the inpatients in the First Hospital of Peking University from Jan.to Jul.2017 were included in this study.The species was identified by using the high resolution matrix-associated laser desorption/ionization time of flight（MALDI-TOF）and the sequencing of 16 sRNA and rpoB was performed.The drug susceptibility test was conducted by using etest test paper.Results Ten strains of nocardia including 4 strains of Nocardia cyriacigeorgica,3 strains of Nocardia abscessus,2 strains of Nocardia brasiliensis and 1 strain of Nocardia farcinica.Among them,4 strains were isolated from the sputum,2 strains were isolated from bronchoalveolar lavage fluid,and other 4 strains were isolated from the fester of lung abscess,skin furuncle,articular cavity fluid and peritoneal drainage respectively.The highest drug sensitivities in 10 strains of nocardia were linezolid,trimethoprim sulfamethoxazole and amikacin,all were 100%;gentamycin and ceftriaxone could reach to90%,imipenem was only 40%,the lowest was ciprofloxacin（0%）and clindamycin（10%）.Conclusion The isolated nocardia are mainly Nocardia cyriacigeorgica and Nocardia abscessus.The most sensitive drugs are trimethoprim sulfamethoxazole,amikacin and linezolid.
作者
周佳
金博
黄磊
孙立颖
肖楠
ZHOU Jia;JIN Bo;HUANG Lei;SUN Liying;XIAO Nan(Department of Clinical Laboratory,Peking University First Hospital,Beijing 100034,China;Department of Clinical Laboratory ,Beijing Tsingh Changgung Hospital ,Beijing 102218 ,China)
出处
《重庆医学》
CAS
2018年第19期2545-2548,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(81502391)
关键词
微生物敏感性试验
诺卡菌
种属鉴定
药敏特征
microbial sensitivity tests
nocardia
species identification
drug susceptibility characteristic
