摘要
为建立水貂阿留申病(AD)抗体间接ELISA检测方法,本研究通过生物信息学预测筛选多个阿留申病毒(ADV)VP2结构蛋白保守的B细胞表位,设计人工诊断抗原肽SD,经原核表达、纯化后作为ELISA的包被抗原,建立了间接ELISA检测方法。结果显示该方法特异性好、敏感性高,与对流免疫电泳(CIEP)方法的符合率达93.2%。利用该方法对我国主要养貂区域的2190份血清样品进行AD血清学调查,结果显示山东、大连、河南、吉林、黑龙江AD阳性率分别为80.41%、82.14%、69.81%、71.24%、78.41%,表明AD在我国已呈高发态势。本研究建立的ELISA方法显著提高了AD阳性检出率,对貂场AD的净化及养貂产业的健康发展具有重要意义。
In order to establish an assay for rapid detection of the antibodies against Aleution disease virus （ADV）, several conserved B cell epitopes of the structural protein VP2 of ADV were screened by bioinformatics prediction, and the indirect ELISA was developed for the antibody detection with the prokaryoticaly expressed and purified artificially designed peptide antigen SD as the coated antigen. The results showed that this method was specific and sensitive, and the coincidence rate was 93.2% with counter immunoelectrophoresis. Moreover, a total of 2,190 serum samples collected from mink farms were tested by the established assay and the positive rates of Aleution disease （AD） were 80.41%, 82.14%, 69.81%, 71.24% and 78.41% in Shandong, Dalian, Henan, Jilin and Heilongjiang provinces, respectively, indicating that AD had a high incidence in these areas. The method had the advantages of high specificity and sensitivity, and obviously improves the positive detection rate, and was of great practical significance for eradication of AD in mink farms and for the healthy development of the mink industry.
作者
孙殿钢
雷连成
黄盼盼
刘洪涛
杨柳枝
刘建方
冯新
孙长江
SUN Dian-gang;LEI Lian-cheng;HUANG Pan-pan;LIU Hong-tao;YANG Liu-zhi;LIU Jian-fang;FENG Xin;SUN Chang-jiang(College of Veterinary Medicine, Jilin University, Changchun 130062, China;Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 402160, China 3. Qingdao Yi Yi He farming and animal husbandry Technology, Qingdao 266100, China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2018年第6期509-513,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然基金(31372447)
