摘要
目的建立HPLC法测定不同产地刺五加中原儿茶酸、紫丁香苷、绿原酸、刺五加苷E和异嗪皮啶。方法采用Welch Materials C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈–0.1%磷酸水,梯度洗脱;体积流量为1.0 m L/min;柱温30℃;检测波长214 nm;进样体积10μL。测定结果采用主成分分析法进行分析。结果 5种成分在测定质量浓度范围内线性关系良好,r均大于0.999 9;平均回收率为99.66%~101.25%,RSD值为1.92%~3.03%;不同产地的11批刺五加中原儿茶酸、紫丁香苷、绿原酸、刺五加苷E和异嗪皮啶的质量分数范围分别为0.004%~0.016%、0.080%~0.152%、0.372%~0.806%、0.057%~0.103%、0.002%~0.010%。主分成分析结果显示,黑龙江产刺五加药材质量较佳。结论该方法快速、简便、准确,多成分测定的质量评价模式可用于刺五加药材的质量控制。
Objective To establish an HPLC method for simultaneous determination of protocatechuic acid, syringing, chlorogenic acid, eleutheroside E and isofraxidin in Acanthopanax senticosus（Rupr. et Maxim.） Harms from different habitats. Methods Separation was carried out on Welch Materials C_（18） column（250 mm × 4.6 μm, 5 μm）. The mobile phase consisted of acetonitrile-0.1% phosphoric acid water with gradient elution. The detection wavelengths were set at 214 nm. The flow rate was 1.0 m L/min, temperature of column was set at 30 ℃, and volume of injection was 10 μL. The result was analyzed by PCA. Results The linear relations of five active components in the range of mass concentration was good with perfect precision with r all more than 0.999 9. The average recovery rates were 99.66% — 101.25%, and RSD was 1.92% — 3.03%. The contents in 11 batch of the A. senticosus from different habitats were calculated, and the results were as following： protocatechuic acid was about 0.004% — 0.016%, syringing was 0.080% — 0.152%, chlorogenic acid was 0.372% — 0.806%, eleutheroside E was 0.057% — 0.103%, and isofraxidin was 0.002% — 0.010%. Conclusion The method is rapid, convenient, and with precision and accuracy, and the multi-component determination method is suitable for the quality control of A. senticosus.
作者
尚海花
王淼
刘颖
郑雅楠
白润
廖茂梁
SHANG Hai-hua;WANG Miao;LIU Ying;ZHEN Ya-nan;BAI Run;LIAO Mao-liang(Shenyang Pharmaceutical University, Shenyang 110016, China;Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China)
出处
《现代药物与临床》
CAS
2018年第6期1324-1328,共5页
Drugs & Clinic
基金
天津市科技计划项目(12ZCDZSY11600
14ZCZDSY00005)
天津市科技支撑计划项目(15ZCZDSY00470)
天津市应用基础与前沿技术研究计划(14JCQNJC13800
15JCYBJC29500)
关键词
刺五加
原儿茶酸
紫丁香苷
绿原酸
刺五加苷E
异嗪皮啶
高效液相色谱
主成分分析
Acanthopanax seuticosus （Rupr. et Maxim.） Harms
protocatechuic acid
syringing
chlorogenic acid
eleutheroside E
isofraxidin
HPLC
PCA
