摘要
目的通过表征和体外相关实验分析选择性激光熔融（SLM）3D打印钛种植体试样表面的生物相容性能,为SLM打印种植体最终应用于临床提供理论基础和实验依据。方法使用SLM方法制备得圆片形钛种植体试样60枚,经不同处理后分为三组：喷砂酸蚀组（S组）、阳极氧化组（SA组）、阳极氧化＋rhBMP-2表面组（SAB组）;场发射扫描电镜（FE-SEM）和原子力显微镜（AFM）观察各组试样的表面形貌和粗糙度,接触角测量仪检测亲水性;SEM观察骨髓间充质干细胞（BMMSCs）在各组试样表面的粘附状态;CCK-8检测BMMSCs在各组试样表面的增殖水平。结果 SEM观察结果显示阳极氧化后的试样SA组表面可见一层均匀密布的二氧化钛纳米管,直径约为100纳米;AFM观察试样表面粗糙度约为300纳米;接触角测量仪测得经表面处理和改性的SA组,SAB组试样具有超亲水性;BMMSCs粘附和增殖实验显示各组试样表面的促细胞粘附和增殖能力SAB组〉SA组〉S组。结论 SLM打印种植体试样未处理组和处理组都具有较好的生物相容性能,阳极氧化与加载BMP-2生长因子处理结合的方法能有效促进BMMSCs的粘附和增殖。
Objective To analyze of the selective laser melting（SLM） printing titanium implant specimens＇ biocompatibility with surface characterization and in vitro cell experiment, to provide theoretical and experimental basis for what the eventual application of 3 D printing implant in clinical therapy. Methods60 SLM printing titanium implant wafer specimens underwent sandblasting polishing, 5% hydrofluoric acid pickling and then ultrasonic cleaning, and drying in fluid N2. 20 pieces were randomly selected and allocated into group S; The remaining 40 specimens were treated by anodic oxidation（AO） for 30 min, flushed by deionized water, and underwent heat treatment at 500℃ for 3 h. 20 pieces of the remaining 40 were randomly selected and allocated into group SA; 0.1% BMP-2 solution was prepared as the surface modification solution. Afterwards, the remaining 20 specimens treated by ultrasonic shock were soaked in the solution for10 s and then allocated as group SAB; Field emission scanning electron microscopy（FE-SEM） and atomic force microscope（AFM） were used to observe the surface topography and roughness of each specimen.Contact angle was adopted for measurement of hydrophilicity; Bone marrow mesenchymal stem cells（BMMSCs） were seeded onto the surface of specimens, and gold was sprayed for observation of the adhesion of BMMSC by SEM; CCK-8 was used to detect the proliferation of the BMMSCs at the surface of each group. Results The SEM pictures showed that the specimens in group SA treated by anodic oxidation had a layer of uniform titania nanotubes on the surface, about 100 nm in diameter. The AFM pictures showed that the surface of group S and group SA had similar roughness values of 300 nm. Contact angle measurement showed the surface of specimens group S and group SA had super hydrophilicity, and specimens in untreated group S also had hydrophilicity. The SEM and CCK-8 kit results showed that the adhesion and proliferation of BMMSCs was better in the group SAB than in group SA and group S. Conclusions SLM printing implant specimens in the untreated group and treatment group both have high biocompatibility, and processing methods such as anode oxidation and load with BMP-2 can effectively promote the adhesion and proliferation of BMMSCs.
作者
严玲玲
郑丽梅
高杰
杨承锋
李严兵
黄文华
YAN Ling-ling;ZHENG Li-mei;GAO Jie;YANG Cheng-feng;LI Yan-bing;HUANG Wen-hua(Guangdong Engineering Research Center for Translation of Medical 3D Printing Application Department of Anatomy, Southern Medical University, Guangzhou Guangdong 510515, China;Stomatological Hospital of Guangzhou Medical University, Guangzhou Guangdong 510140, China)
出处
《中国临床解剖学杂志》
CSCD
北大核心
2018年第3期313-318,共6页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金面上项目(21773199)
广东省科技计划项目(2016B090925001,2016B090917001)
南方智谷引进创新团队项目(2015CXTD05)
