摘要
该文应用色谱-质谱技术对加味小柴胡汤抗胃溃疡有效物质部位进行血清药物化学研究。通过小鼠无水乙醇致胃溃疡模型考察了全方和有效部位组方抗胃溃疡的药效作用,两者均能明显降低无水乙醇致小鼠溃疡;通过气相-质谱法,检测挥发性部位的化学成分和灌胃给药无水乙醇诱导的大鼠胃溃疡模型后的入血成分,共分析鉴定了63个化合物,约占挥发油总量的93%以上。其中α-姜黄烯23.51%,姜烯11.96%,莪术呋喃烯9.56%,β-倍半水芹烯6.54%,莰烯4.77%,为加味小柴胡汤挥发性物质部位的主要成分,在抗胃溃疡的含药血清中检测到6个原形成分;通过液相-质谱法,正负离子模式同时检测生物碱物质部位提取物化学成分和灌胃给药无水乙醇诱导的大鼠胃溃疡模型后的入血成分,共分析鉴定出17个化合物,在抗胃溃疡的含药血清中检测到6个原形成分,及3,5-O-feruloylquinic acid,巴马汀2个代谢成分。该研究明确了加味小柴胡汤抗胃溃疡的主要有效化学成分,阐明了其药效物质基础,为该经典方剂治疗胃溃疡的创新性临床应用提供科学实验依据,并为进一步的中药新药研制提供基础。
To conduct the studies on serum pharmacochemistry of effective parts of modified Xiaochaihu Tang for treatment of gastric ulcer by using chromatography-mass spectrometry. Absolute ethyl alcohol induced-gastric ulcer model of mouse was used in this study to investigate the pharmacology of modified Xiaochaihu Tang and its effective parts. Both groups could significantly decrease the absolute ethyl alcohol induced-ulcer. Gasphase-mass spectrometry（ GC-MS） was used to detect chemical compositions of volatile fractions and the drug components after gastric administration. A total of 63 compounds were identified in extracts,accounting for more than 93% of the all volatile oil,including 23. 51% α-curcumene,11. 96% zingiberene,9. 56% curzerene,6. 54% β-sesquinene,4. 77% camphene,and 6 prototype components were also detected in serum for gastric ulcer model. In liquid chromatography-mass spectrometry（ LC-MS）,a total of 17 compounds were identified in extracts,6 prototype components and 2 metabolites（ 3,5-O-feruloylquinic acid and palmatine） were obtained in serum. In a conclusion,this study provides an important scientific basis for identifying the active ingredients in modified Xiaochaihu Tang,and also helps to reveal the pharmacological effect of modified Xiaochaihu Tang for treatment of gastric ulcer.
作者
陈雪剑
杨梦玲
刘蔚
尤朋涛
周爱军
刘焱文
陈新
CHEN Xue-jian;YANG Meng-ling;LIU Wei;YOU Peng-tao;ZHOU Ai-jun;LIU Yan-wen;CHEN Xin(Resource and Chemistry of Chinese Medicine Hubei Provincial Key Laboratory, Hubei University of Chinese Medicine, Wuhan 430065, China;Dongguan Hospital of Traditional Chinese Medicine, Dongguan 523000, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第8期1692-1700,共9页
China Journal of Chinese Materia Medica
基金
广东省东莞市科技局项目(东科[2014]130号)
湖北中医药大学科研启动基金项目(5114-100714)
关键词
加味小柴胡汤
胃溃疡
有效物质部位
血清药物化学
modified Xiaochaihu Tang
gastric ulcer
effective chemical position
serum pharmacochemistry
