摘要
目的研究钽颗粒对成骨细胞增殖的影响,并初步探索其机制。方法将不同浓度的微粒钽和纳米钽颗粒分别与成骨细胞共培养,CCK8法检测6、12、24、48 h时细胞的OD值。依据CCK8结果,筛选促增殖作用最明显的组别,用Western blot、激光共聚焦显微镜、透射电镜检测细胞自噬水平。最后,结合自噬诱导剂和自噬抑制剂进一步验证自噬在纳米钽促增殖反应中的作用。结果 100ng/mL微米钽处理组促增殖作用明显,但未检测到细胞自噬;20μg/mL纳米钽处理组促增殖作用明显,可检测到明显的自噬;自噬抑制剂可明显抑制纳米钽处理细胞的增殖。结论纳米钽可通过诱导细胞自噬发挥促增殖作用,微米钽可能通过其他非自噬途径促进成骨细胞增殖。
Objective To investigate the effect of tantalum particles on the proliferation of osteoblasts andexplore its mechanism. Methods Mouse osteoblasts MC3T3-E1 were co-cultured with micro-tantalum particles（micro-Ta）and Nano-tantalum particles（nano-Ta）of different concentrations respectively. CCK-8 assay was usedto measure the cell viability at 6,12,24 and 48h. According to the result of CCK-8 the group with the most prolif-erative effect was screened and the level of autophagy was detected by using Western blot,laser confocal microsco-py and transmission electron microscopy（SEM）. Finally,to verify the role of autophagy in pro-proliferation effectof nano-Ta,the OD value was measured repeatedly in combination with autophagy inducer and inhibitor. Results 100ng/m L micro-Ta treated groups had obvious proliferative effect but autophagy was not detected. 20μg/mL nano-Ta treated groups had obvious proliferative effect and autophagy was detected. CCK-8 assay revealed that autophagyinhibitor can significantly inhibited cell proliferation of nano-Ta treated group. Conclusion Nano-Ta could pro-mote cell proliferation by inducing autophagy,and micro-Ta may promote osteoblast proliferation through other non-autophagy pathway.
作者
康成容
李梁
李张维
周折冲
周倩冰
潘宣
KANG Chengrong;LI Liang;LI Zhangwei;ZHOU Zhechong;ZHOU Qianbing;PAN Xuan(Department of Stomatology the First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510080,China)
出处
《实用医学杂志》
CAS
北大核心
2018年第10期1618-1623,共6页
The Journal of Practical Medicine
基金
广东省科技发展专项基金(公益研究与能力建设方向)资助(编号:2016A020215154)
关键词
钽颗粒
成骨细胞
增殖
自噬
tantalum particles
osteoblast
proliferation
autophagy
