摘要
研究超临界CO_2流体萃取花椰菜种子油,利用响应面法优化油脂萃取工艺,并分析花椰菜种子油脂肪酸构成。采用Plackett-Burman试验设计,从7个因素中筛选得到影响超临界CO_2萃取花椰菜种子油提取率的3个主要因素,分别是萃取压强、萃取温度、萃取时间。基于最陡爬坡试验设计,得到工艺参数最佳值区域。通过Box-Behnken试验开展响应面分析,得到提取率数学模型,方差分析表明该回归模型有效、可靠。确定最佳萃取条件是:萃取压强为34 MPa、萃取温度为44℃、萃取时间为91 min,预测提取率为92.17%,实际提取率为92.08±0.71%。采用GC-MS分析花椰菜种子油脂肪酸组成,结果表明其脂肪酸种类与萝卜种子油、菜籽油极为相似,但含量存在显著差异。该油品中不饱和脂肪酸含量为93.81%,单不饱和脂肪酸含量为84.65%。
Response surface methodology was used to optinfize the extraction process of oil from cauliflower seed by Supercritical CO： ,and the fatty acid compositions of oil were analyzed. Three factors play- the important roles in the extraction process ,including extraction pressure, temperature and time, selected based on Plackett-Burman design. Then the optimal region was obtained by Stee- pest ascent path. The feasible and effective regression model were deternfined with Box-Behnken design and response surface analy- sis. As a result the optimal conditions were as follow： extraction pressure 34MPa, extraction temperature 44℃, extraction time 91 nfin. Under such conditions, the actual and predicted extraction rate were 92.08 ± 0. 71% and 92.17%, respectively. The gas chromatography-nlass spectrometry was then used to analyze the extracted oil. These results indicated that the fatty- acid substances were similar in radish seed oil and colleseed oil, but the relative contents of most compounds were significant difference. And the contents were 93. 81% and 84. 65 % for unsaturated and monounsaturated fatty acid.
作者
张杰
冯春荣
谭雪梅
贺有周
叶梅
文莉
王瑞琪
ZHANG Jie;FENG Chun-rong;TAN Xue-mei;HE You-Zhou;YE Mei;WEN Li;WANG Rui-qi(College of Environment and Resources, Chongqing Technology- and Business University, Chongqing 400067, China;Chongqing Engineering Research Center for Processing, Storage and Transportation of Characterized Agro-Products, Chongqing 400067, China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2018年第5期751-757,共7页
Chemical Research and Application
基金
重庆市前沿与应用基础研究计划一般项目(cstc2016jcyj A0147)资助
重庆市社会事业与民生保障科技创新专项(cstc2017shmsxdny100003)资助
重庆市教委科学技术研究项目(KJ1500622)资助
重庆工商大学科研启动经费项目(2015-56-05)资助
重庆工商大学校内青年基金项目(1552017)资助
关键词
响应面
花椰菜种子油
脂肪酸
超临界CO2萃取
response surface methodology
cauliflower seed oil
fatty acid
supercritical CO2 extraction
