摘要
建立了UHPLC-MS/MS同时测定大鼠血浆中的汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量的方法。以芹菜苷为内标,采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm),乙腈和0.1%甲酸溶液为流动相,流速为0.2 mL·min-1。结果表明,汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷线性范围为1~1 000 ng·mL^(-1)(R2>0.99),最低定量浓度为1 ng·mL^(-1),提取回收率、基质效应均符合生物样品分析要求。建立的方法灵敏、准确,可用于大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷的测定及其药代动力学研究。
A rapid ultra-high performance liquid chromatography tandem mass spectrometry（UHPLC-MS/MS） method was developed for the simultaneous analysis of wogonin,4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma.Apiin was selected as the internal standard.The analysis was carried out on an ACQUITY UPLC HSS T3 column（2.1 mm×100 mm,1.8 μm）,with acetonitrile and 0.1% formic acid aqueous solution,gradient elution.The flow rate was 0.2 mL·min^（-1）.Linear responses were obtained for wogonin,4-dicaffeoylquinic acid and chrysin-7-O-Beta-Dglucoronide ranging from 1 to 1 000 ng·mL^（-1）.The correlation coefficients were better than 0.99.Both recovery rates and matrix effects met the requirement of biological sample analysis.The established UHPLC-MS/MS quantitative analysis method shows high sensitivity,accuracy and satisfying recovery,and can be utilized to determine the pharmacokinetic data of wogonin,4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma.
作者
李翠
罗孟雄
聂其霞
臧琛
王国华
罗赣
张保献
LI Cui;LUO Meng-xiong;NIE Qi-xia;ZANG Chen;WANG Guo-hua;LUO Gan;ZHANG Bao-xian(China Academy of Chinese Medical Science, Beijing 100700, China;Institute of Chinese Materia Medica China Academy of Chinese Medical Science. Beijing 100700, China;Shandong University of Traditional Chinese Medicine, Jinan 250355, China)
出处
《山东科学》
CAS
2018年第2期14-20,共7页
Shandong Science
基金
全国中药特色技术传承人才培训项目(L2016002)
